Induction of IgA and sustained deficiency of cell proliferative response in chronic hepatitis C  

作　　者：Yalena Amador-Caizares Liz Alvarez-Lajonchere Ivis Guerra Ingrid Rodríguez-Alonso Gillian Martínez-Donato Julián Triana Eddy E González-Horta Angel Pérez Santiago Dueas-Carrera 

机构地区：[1]Centro de Ingeniería Genética y Biotecnología,Ave 31,e/ 158 and 190,Playa,P.O. Box 6162,Havana,Cuba

出　　处：《World Journal of Gastroenterology》2008年第44期6844-6852,共9页世界胃肠病学杂志（英文版）

基　　金：Supported by Grant from Pan American Health Organization, held by Dr. Santiago Dueas-Carrera

摘　　要：AIM： In the present study, antibody and peripheral blood mononuclear cells （PBMC） proliferative responses against hepatitis C virus （HCV） antigens were evaluated in HCV chronically infected patients. METHODS： Paired serum and PBMC samples were taken six months apart from 34 individuals, either treated or not, and tested by enzyme-linked immunosorbent assay （ELISA） and carboxyfluorescein succinimidyl ester staining. RSULTS： Over 70% of the patients showed specific IgG and IgM against capsid, E1 and NS3, while HVR-1 was recognized by half of the patients. An increase in the levels of the anti-capsid IgM （P = 0.027） and IgG （P = 0.0006） was observed in six-month samples, compared to baseline. Similarly, a significantly higher percent of patients had detectable IgA reactivity to capsid （P = 0.017） and NS3 （P = 0.005） after six months, compared to baseline. Particularly, IgA against structural antigens positively correlated with hepatic damage （P = 0.036）. IgG subclasses evaluation against capsid and NS3 revealed a positive recognition mediated by IgG1 in more than 80% of the individuals. On the contrary, less than 30% of the patients showed a positive proliferative response either of CD4＋ or CD8＋ T cells, being the capsid poorly recognized. CONCLUSION： These results confirm that while the cellular immune response is narrow and weak, a broad and vigorous humoral response occurs in HCV chronic infection. The observed correlation between IgA and hepatic damage may have diagnostic significance, although it warrants further confirmation.AIM:In the present study,antibody and peripheral blood mononuclear cells (PBMC) proliferative responses against hepatitis C virus (HCV) antigens were evaluated in HCV chronically infected patients. METHODS:Paired serum and PBMC samples were taken six months apart from 34 individuals,either treated or not,and tested by enzyme-linked immunosorbent assay (ELISA) and carboxyfluorescein succinimidyl ester staining. RESULTS:Over 70% of the patients showed specifi c IgG and IgM against capsid,E1 and NS3,while HVR-1 was recognized by half of the patients. An increase in the levels of the anti-capsid IgM (P = 0.027) and IgG (P = 0.0006) was observed in six-month samples,compared to baseline. Similarly,a signifi cantly higher percent of patients had detectable IgA reactivity to capsid (P = 0.017) and NS3 (P = 0.005) after six months,compared to baseline. Particularly,IgA against structural antigens positively correlated with hepatic damage (P = 0.036). IgG subclasses evaluation against capsid and NS3 revealed a positive recognition mediated by IgG1 in more than 80% of the individuals. On the contrary,less than 30% of the patients showed a positive proliferative response either of CD4+ or CD8+ T cells,being the capsid poorly recognized. CONCLUSION:These results confi rm that while the cellular immune response is narrow and weak,a broad and vigorous humoral response occurs in HCV chronic infection. The observed correlation between IgA and hepatic damage may have diagnostic significance,although it warrants further confi rmation.

关 键 词：Hepatitis C Antibody response LYMPHOPROLIFERATION Core ENVELOPE 

分 类 号：R512.6[医药卫生—内科学]