水稻中一个谷胱甘肽转移酶基因的克隆、表达和酶活性分析(英文)  

Molecular Cloning,Expression and Activity Analysis of a Glutathione S-transferase in Rice

作　　者：胡廷章[1,2] 黄小云[2] 肖国生[2] 屈霄霄[1] 

机构地区：[1]重庆大学生物工程学院,重庆400044 [2]重庆三峡学院生物系,重庆404000

出　　处：《云南植物研究》2008年第6期688-692,共5页Acta Botanica Yunnanica

基　　金：国家自然科学基金(30771464);重庆市教育委员会科学技术研究项目(KJ071112);重庆三峡学院资助项目(2007-Sxxyyb-04)

摘　　要：OsGSTL1是位于水稻3号染色体上的一个λ类谷胱甘肽转移酶基因,由8个内含子和9个外显子组成,编码一个由243个氨基酸组成的多肽链。将OsGSTL1克隆到酵母表达载体pYTV上,转化大肠杆菌,然后再转化酵母菌PEP4。Western印迹分析表明外源OsGSTL1基因在转基因酵母中表达,分析半乳糖诱导表达的酵母粗提液的谷胱甘肽转移酶活性表明:转基因酵母的谷胱甘肽转移酶较非转基因酵母和未诱导的酵母高,说明OsGSTL1在转基因酵母中受半乳糖的诱导表达,具有谷胱甘肽转移酶活性。OsGSTLl is a glutathione S-transferase （GSTs, EC 2.5.1.18）, lambda class gene in chromosome 3 of rice （ Oryza sativa L. ）. It includes 8 introns and 9 exons, and encodes a protein of 243 amino acid residues. The OsGSTLl gene was cloned into pYTV vector and was transformed into yeast strain PEP4. Western blot analysis showed the exogenous OsGSTLI was expressed in transformed yeast. GST activity of crude extracts of yeast showed the OsGSTLl transgenic yeast had higher levels of GST activities than control yeasts, which demonstrate the OsCSTLl has glutathione S-transferase activity.

关 键 词：谷胱甘肽转移酶 水稻 Western印迹分析 GST活性 

分 类 号：S511[农业科学—作物学] Q943.2[生物学—植物学]