青春期龈炎患者牙周可疑致病菌的检测  

Bacteriological analysis of subgingival plaque in adolescents

作　　者：吕绳漪[1] 时清[1] 杨圣辉[2] 

机构地区：[1]首都医科大学口腔医学院儿童口腔科, 100050 [2]首都医科大学口腔医学院口腔医学研究所, 100050

出　　处：《中华口腔医学杂志》2008年第12期737-740,共4页Chinese Journal of Stomatology

摘　　要：目的研究青春期龈炎患者龈下菌斑中牙周致病菌的变化特点及其与临床症状的相关性。方法选择2006年6至10月于首都医科大学口腔医学院儿童口腔科及牙周科门诊就诊的11至17岁患者共54例，采集龈下菌斑样本108个，其中牙龈炎组（G组）30例（60个样本，牙龈轻度炎症20个样本，中度炎症19个样本，重度炎症21个样本），健康对照组（H组）15例（30个样本），慢性牙周炎对照组（P组）9例（18个样本），用细菌培养法检测产黑色素菌群、伴放线放线杆菌、放线菌、具核梭杆菌，同时记录取样牙牙周探诊深度（probingdepth，PD）、牙龈指数（gingivalindex，GI）、出血指数（bleedingindex，BI）、附着丧失（mtachmentloss，AL）。结果在H、G、P3组中，产黑色素菌群的检出率分别为：3％、30％和100％；具核梭杆菌的检出率分别为：30％、68％和94％，3组相比差异有统计学意义（P〈0．01）。牙龈轻、中度炎症组具核梭杆菌和放线菌的菌落计数[分别为（3．8±0．7）和（5．3±0．9）]较H组[分别为（3．1±0．4）和（4．2±0．8）]明显增加（P〈0．叭）；牙龈重度炎症组产黑色素菌群和具核梭杆菌菌落计数[分别为（4．7±1．2）和（4．4±0．8）]较轻度炎症组[分别为（3．1±0．5）和（3．8±0．7）]明显增加（P〈0．01）；而P组产黑色素菌群、具核梭杆菌、伴放线放线杆菌的菌落计数[分别为（6．6±1．0）、（5．5±1．0）和（4．2±1．7）]均比牙龈重度炎症组[分别为（4．7±1．2）、（4．4±0．8）和（2．6±0．9）]明显增加（P〈0．01）。产黑色素菌群、具核梭杆菌、伴放线放线杆菌的检出率和菌落计数均与BI、PD和AL呈正相关（P〈0．01）。结论重度牙龈炎时，牙周致病菌已明显增加，提示牙周组织存在进一步破坏的危险，临床上应给予足够重视。Objective To investigate the changes in characteristics of periodontal pathogens in subgingival plaque of patients with puberty gingivitis and its relevance with clinical symptoms. Methods A total of 108 subgingival plaque samples were collected from 30 patients with puberty gingivitis（ Guoup G）, 9 cases of chronic periodontitis（ Group P） and 15 healthy controls（ Group H）. The age of the 54 participants was from 11 to 17. The black-pigmented bacteria（BPB）, Fusobacterium nucleatum （Fn）, Actinobacillus actinomycetemcomitans （Aa）, Actinomyces were detected using bacterial culture. The probing depth （PD）, gingival index （GI） , bleeding index （BI） and attachment loss （AL） were also recorded. Resulls In all these three groups, the detection rates of black-pigmented bacteria were： 3%, 30% and 100% ; Fn were： 30% , 68% and 94% , statistically significantly different （ P 〈 0. 01 ）. The lgCFU/ml of black-pigmented bacteria and Actinomyces was higher in mild-moderate group [ （ 3.8 ± 0. 7 ） and （ 5. 3 ± 0. 9 ） ] than in Group H （P 〈 0. 001 ）. The lgCFU/ml of black-pigmented bacteria and Fn significantly was higher in severe inflammation group [ （4. 7 ± 1.2 ） and （ 4. 4 ± 0. 8 ） ] than in the mild-moderate group （ P 〈 0. 01 ）. The lgCFU/ml of black-pigmented bacteria, Fn and Aa was higher in severe gingivitis group [ （ 6. 6 ± 1.0 ）, （ 5.5 ± 1.0 ） and （4. 2 ± 1.7 ） ] than in mild gingivitis group （ P 〈 0.01 ）. The detection rate and lgCFU/ml of black-pigmented bacteria, Fn and Aa were both positively correlated with BI, PD and AL. Coudusions In the stage of severe gingivitis, the periodontal pathogens increased markedly, suggesting that risk of further destruction of periodontal tissue may exist.

关 键 词：牙龈炎 牙菌斑 细菌学技术 

分 类 号：R686[医药卫生—骨科学]