压力作用下大鼠颞下颌关节滑膜细胞超微结构的变化  

Effect of hydrostatic pressure on ultrastructure of synovial fibroblasts from rat temporomandibular joint

作　　者：吴梦婕[1] 冯剑颖[1] 谷志远[2] 

机构地区：[1]浙江大学医学院附属口腔医院正畸科,杭州310006 [2]浙江大学医学院附属口腔医院口腔颌面外科,杭州310006

出　　处：《中华口腔医学杂志》2008年第12期754-755,共2页Chinese Journal of Stomatology

基　　金：国家自然科学基金（30471900）

摘　　要：目的探讨在应力作用下颞下颌关节（temporomandibularjoint，TMJ）滑膜细胞超微结构的变化及意义。方法大鼠TMJ滑膜细胞原代培养，透射电镜下观察不同静水压力（0、30、60、90kPa）作用12h后细胞超微结构的改变。结果大鼠30kPa压力下细胞超微结构变化不明显；60kPa压力下细胞胞质紧密围绕胞核，呈新月形，线粒体轻度肿胀；90kPa压力下，核内结构溶解，胞质内空泡形成，有类似包涵体的“凋亡小体”存在，部分核膜结构不完整。结论滑膜细胞在压力作用下超微结构会发生凋亡样变化，且这种变化程度与压力大小有关。Objective To investigate the role of apoptosis in the remodeling of temporomandibular joint （TMJ） under pressure. Methods Synovial fibroblasts obtained from rat temporomandibular joint were subjected to different hydrostatic pressure for 12h. Changes of ultrastructure were observed by transmission electron microscope. Results At 30 kPa, the uhrastructure of synovial fibroblasts showed no obvious changes. At 60 kPa, the chromatin was condensated, the karyon took on crescent and the mitochondria seemed varicose. At 90 kPa, the apoptosis-like body was wrapped by membrane and embedded in the high density chromatin. Conclusions Apoptosis-like change took place in uhrastructure of synovial fibroblasts under hydrostatic pressure, and the degree of the change was related to the hydrostatic pressure exerted.

关 键 词：颞下颌关节 滑膜细胞 应力 

分 类 号：R686[医药卫生—骨科学]