辛硫磷对大鼠生殖内分泌系统的影响  

Effects of phoxim on reproductive and endocrine systems of male rats

作　　者：胡静熠[1] 王心如[2] 

机构地区：[1]南京医科大学附属南京妇幼保健院,210004 [2]南京医科大学江苏省共建教育部现代应用毒理重点实验室

出　　处：《江苏医药》2008年第12期1258-1261,共4页Jiangsu Medical Journal

基　　金：国家"九五"医学科技攻关项目(96-906-04-11);国家攀登项目子课题(2000504)

摘　　要：目的研究辛硫磷对雄性大鼠生殖内分泌系统的影响。方法将每日不同剂量辛硫磷(5.9、29.4、147.0)mg/kg分别对雄性成年SD大鼠连续灌胃染毒15 d和30 d,应用RIA法测定血清卵泡刺激素(FSH)、黄体生成素(LH)、睾酮(T)和睾丸匀浆中睾酮(T)的水平,同步测定睾丸标志酶酸性磷酸酶(ACP)、γ-谷氨酰转移酶(γ-GT)的活性,并采用精子头计数法观测每日精子生成量(Spr)的变化。结果与对照组比较,染毒大鼠15 d时,血清LH水平5.9 mg/kg,各染毒组表现为显著升高(P<0.05);血清FSH的水平随染毒剂量增加而升高,各染毒组均明显高于对照组(P<0.01);血清T水平随染毒剂量的增加呈现为升高的趋势,在147.0 mg/kg剂量组差异有统计学意义(P<0.05)。染毒至30 d时,血清中LH水平在147.0 mg/kg剂量组差异有统计学意义(P<0.05);FSH在≤29.4 mg/kg剂量组表现有统计学意义(P<0.05)。ACP各染毒组有统计学意义(P<0.01)。γ-GT的活性在≥29.4 mg/kg剂量组范围均有明显差异(P<0.01)。Spr与染毒剂量有明显的剂量依赖关系,在≥29.4 mg/kg剂量范围Spr显著减少(P<0.01)。结论辛硫磷对雄性大鼠有明显的生殖毒性,可影响其血清及睾丸性激素水平和酶活性,导致精子生成障碍。Objective To evaluate the impairment of phoxim on reproductive and endocrine systems.Methods Different doses of phoxim（0,5.9,29.4,147）mg/kg were orally given to adult male SD rats ones a day for 15 and 30 days,respectively.The serum follicle stimulating hormone（FSH）,luteinizing hormone（LH）,testosterone（T） and testis homogenate T were determined by radioimmunoassary.The activity of testicular marked enzymes such as acid phosphatases（ACP） and gamma-glutamyl transpeptidase（γGT） were examined,and sperm head counts were measured to explain the changes of daily sperm prodction（Spr）.Results On the 15th day,the serum（LH） raised in 5.9mg/kg group（P〈0.05）.Significant difference of serum FSH was found in all groups（P〈0.01）.T level in serum 147.0mg/kg group（P〈0.05） was markedly altered compared with that in control group,but homogenate T level tended towards decline in the treated groups.On the 30th day,serum LH in 147.0mg/kg group,and FSH in the doses of ≥5.9mg/kg groups were markedly differed from those in control group（P〈0.05）.Activity of ACP was markedly decreased in all groups（P〈0.01）,and γ-GT was significantly changed in all treated groups on the 15th day in the doses of ≥29.4mg/kg groups（P〈0.01）.Phoxim caused dose-dependent reduction in sperm head counts and daily sperm production,which markedly reduced in the doses of ≥29.4mg/kg groups（P〈0.01）.Conclusion Phoxim can injure spermatogenic epithelium,sertoli cell and leydig cell.Phoxim may exert a direct inhibitory action on the testis and affect the paracrine modulation of spermatogenesis,which induces the disfunction of reproductive and endocrine system and thus subsequent spermatogenetic impairment.

关 键 词：辛硫磷 性激素 精子发生 

分 类 号：R994[医药卫生—毒理学]