滑桃树内生真菌Fusarium sp.2TnP1-2次生代谢产物研究(英文)  

Secondary metabolites produced by Fusarium sp.2TnP1-2,an endophytic fungus from Trewia nudiflora

作　　者：杜芝芝[1] 宋成芝[1,2] 郁步竹[1] 罗晓东[1] 

机构地区：[1]中国科学院昆明植物研究所,云南昆明650204 [2]昭通师范高等专科学校化学系,云南昭通657000

出　　处：《中国药物化学杂志》2008年第6期452-456,共5页Chinese Journal of Medicinal Chemistry

基　　金：supported by National Nature Science Foundation of China(No.30400038);the “XiBuZhiGuang” Project of Chinese Academy of Sciences

摘　　要：目的对滑桃树内生真菌Fusariumsp.2TnP1-2的抗菌活性次生代谢产物进行研究。方法采用硅胶柱色谱、Sephadex LH-20、制备性薄层色谱等对次生代谢产物进行分离纯化。根据理化性质和波谱分析进行结构鉴定。利用纸片扩散法对这些化合物的抗真菌及抗细菌活性进行测试。结果从该菌株PDA培养基发酵产物中分离得到3个化合物,分别鉴定为:trichosetin(-Ndemethyl equisetin,1),lateritin(4-methyl-6-(1-methylethyl)-3-phenylmethyl-1,4-perhydrooxazine-2,5-dione,2),5α,6-αepoxy-24(R)-methylcholesta-7,22-dien-3-βol(3)。结论3个化合物均为首次从该真菌的代谢物中分离得到,trichosetin和lateritin具有抗金黄色葡萄球菌活性。Aim To study antifungal and antibacterial secondary metabolites of Fusarium sp. 2TnP1-2, an endophytic fungus isolated from Trewia nudiflora. Methods PDA fermentation extract was isolated by bio-assay guided fractionation and different column chromatography methods including silica gel column, Sephadex LH-20 column and preparative thin layer chromatography. Structures of these compounds were identified on the basis of spectroscopic analysis of 1D, 2DNMR, MS and comparison of chemical and physical data with authentic samples reported in literatures. The antibacterial and antifungal activities of the isolated compounds were measured using the disc diffusion method. Results Three compounds were isolated and their structures were determined to be trichosetin （ N-demethyl equisetin, 1 ）, lateritin （4-methyl-6-（ 1-methylethyl）-3- phenylmethyl-1,4-perhydrooxazine-2, 5-dione, 2）, 5α, 6α-epoxy-24 （ R ）-methylcholesta-7, 22-dien-3β-ol（3）. Conclusion All the compounds were obtained from Fusarium for the first time. Trichosetin and lateritin possess antibacterial activity against Staphylococcus aureus.

关 键 词：滑桃树 镰刀菌属 抗菌 tetramicacid 

分 类 号：R284[医药卫生—中药学]