射干抗病毒注射液统一化HPLC指纹图谱的评估  

Evaluation on normalized HPLC fingerprints of Shegan Antiviral Injection

作　　者：孙国祥[1] 宋杨[1] 胡玥珊[1] 毕开顺[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《中成药》2008年第12期1717-1720,共4页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金重点研究计划资助项目(90612002)

摘　　要：目的:建立射干抗病毒注射液(射干、金银花、茵陈、柴胡等)及其单味药材和中间体的统一化HPLC指纹图谱,用25个相对统一化特征参数进行评价。方法:采用RP-HPLC法分别建立了射干抗病毒注射液各单味药材、中间体和制剂的对照指纹图谱,用"中药色谱指纹图谱超信息特征数字化评价系统3.0"软件计算11个对照指纹图谱的25个相对统一化特征参数及其统一化指纹图谱。结果:所建立的S4茵陈和S7板蓝根的HPLC指纹谱图(HPLC-FP)最理想,S2金银花、S5柴胡、S6蒲公英、S8大青叶,S10中间体Ⅱ和S11制剂的HPLC-FP也较为合理。结论:25个相对统一化特征判据参数能够准确、真实、直观地揭示射干抗病毒注射液HPLC对照指纹图谱好坏及其统一化特征。AIM： To establish the normalized chromatographic fingerprints （NCFP） of Shegan Antiviral Injectionl （Rhizoma Belamcandae, Flos Lonicerae japonicae, Herba Artemisiae scopariae, Radix Bupleuri, etc. ） （SGKBDI） and to evaluate them by using the 25 relatively normalized parameters. METHODS： The chromatographic fingerprints of the eight herbal drugs, two intermediates and preparation of SGI were determined by RPHPLC. The 11 chromatographic fingerprints were evaluated by 25 relatively normalized index calculated by the software of Digitalized Evaluation System of Super-information Characteristic for Traditional Chinese Medicine Fingerprints 3.0. RESULTS ： The best fingerprints were S4 Herba Artemislae Scopariae and S7 Radix Isatidis, the next were S2 Flos Lonicerae, S5 Radix Bupleuri, S6 Herba Taraxaci, S8 Folium Isatidis, S10 intermediat 11 and S11 Sgkbdi. CONCLUSION： The 25 characteristic parameters can be used to objectively, authentically and thoroughly reveal the separation of chromatographic fingerprints and its normalized characteristics of the NCFP of SGKBDI.

关 键 词：射干抗病毒注射液 统一化指纹图谱 色谱指纹图谱定点指数Fa/t 色谱指纹图谱定信号指数FVa 

分 类 号：R927.1[医药卫生—药学]