白英水提物对胃癌SGC-7901细胞凋亡与基因bcl-xl/bid表达的影响  

Influence of Solanum lyratum Thunb extract on apoptosis and the expression of bcl-xl/bid genes in human stomach cancer SGC-7901 cells

作　　者：余乐涵[1] 许宝华[2] 吴剑[1] 李华[1] 涂硕[1] 万福生[1,2] 

机构地区：[1]南昌大学医学院生物化学与分子生物学教研室,江西南昌330006 [2]南昌大学医学院实验动物科学部,江西南昌330006

出　　处：《中成药》2008年第12期1744-1748,共5页Chinese Traditional Patent Medicine

基　　金：江西省科技攻关重点项目(060004)

摘　　要：目的:观察白英水提物对胃癌SGC-7901细胞凋亡及基因bcl-xl、bid表达的影响。方法:常规法制备白英水提物,实验分正常对照组、白英处理组。白英处理组加入白英水提物终浓度分别为12.5mg/mL、25mg/mL和50mg/mL。流式细胞仪检测细胞凋亡,半定量RT-PCR和免疫化学分析细胞中bcl-xl、bid基因表达。结果:与正常对照组比较,白英处理组SGC-7901细胞增殖有显著性下降,细胞凋亡显著性增多(P<0.05);基因bcl-xl mRNA和Bcl-xl蛋白表达降低、bidmR-NA和Bid蛋白表达升高(P<0.05),并随白英浓度增加而加强。结论:白英水提物能诱导人胃癌SGC-7901细胞凋亡和抑制增殖,且有一定的量效关系,其作用机制可能与调控bcl-xl、bid基因表达有关。AIM： To investigate the effects of Solanum lyratum Thunb Extract （SLT） on apoptosis and the expression of bcl-x1, bid genes in human stomach cancer SGC-7901 cells. METHODS： Dried whole herbs of Solanum lyratum Thunb were extracted by boilingdistilled water. Human stomach cancer SGC-7901 cells were randomly divided into the control group and SLT-treated groups （ 12.5 mg/mL, 25 mg/mL, 50 mg/mL）, cell apoptosis rate wase determined by flow cytometry. Expression of bcl-x1 and bid mRNA were detected by semi-quantitive RT-PCR.Expression level of Bcl-x1 and Bid proteins were detected by two-step immunhistochemical staining. RESULTS： Compared with control group, the proliferation inhibitory rate and apoptosis rate increased obviously （P 〈 0.05）. The expression of bcl-xl mRNA and Bcl-x1 protein obviously decreased respectively （P 〈 0.05）. The expression of bid mRNA and Bid protein obviously increased respectively （P 〈 0.05）. CONCLUSION ： SLT can induce apoptosis and inhibit proliferation of the Human stomach cancer SGC-7901 cells, and display effect in a dose-dependent manner by regulating expression of bcl-x1 and bid genes.

关 键 词：白英 SGC-7901细胞 凋亡 bcl—xl/bid 基因表达 

分 类 号：R285.6[医药卫生—中药学]