高效液相色谱法测定小儿润肺口服液中黄芩苷含量  

Determination of baicalin in Xiaoer Runfei Oral liquid by HPLC

作　　者：姜韧[1] 刘刚[1] 张华[1] 常华[1] 谭生建[1] 

机构地区：[1]中国人民解放军第306医院药学部,北京100101

出　　处：《药学实践杂志》2008年第6期435-437,共3页Journal of Pharmaceutical Practice

摘　　要：目的:建立HPLC测定小儿润肺口服液中黄芩苷含量的方法。方法:色谱柱用十八烷基硅烷键合硅胶为固定相(Agilent ZORBAX Eclipse XDB-C18,4.6 mm×150 mm,5μm)。流动相为甲醇-水-磷酸(38:62 :0.5),流速:1 mL/min,检测波长278 mm。结果:黄芩苷的保留时间约为20.6 min,与相邻峰的分离度大于1.5。以峰面积(X)对进样浓度(Y,μg/mL)线性回归,回归方程:Y=0.060 47X+1.131,r=0.999 9,线性范围:62.84～314.2μg/mL,加样回收率为103.3%,RSD为0.43%。结论:本法测定结果准确,且操作简便,精密度好,可以用于小儿润肺口服液中黄芩苷的含量测定。Objective： To develop an HPLC quantitative method for the determination of baicalin in Xiaoer Runfei Oral liquid. Methods：HPLC system consisted an Agilent C18 column（ ZORBAX Eclipse XDB-C18,4.6 mm× 150 mm,5 μm） and a mixture of methanol-water-phosphoric acid （ 38 ： 62 ： 0.5 ） as mobile phase. The flow rate was 1.0 mL/min and was monitored at 278 nm. Results ：The retention time of baicalin was 20.6 rain. The regress equation for baicalin was Y = 0. 060 47X ＋ 1. 131, r = 0. 999 9, and the linear range was 62.84- 314.2 μg/mL. The average recovery of baicalin was （n = 6）103.3% , RSD 0. 43% . Conclusion ：The method was sensitive ,time-saving and accurate.

关 键 词：高效液相色谱 小儿润肺口服液 黄芩苷 

分 类 号：R927.2[医药卫生—药学]