乙型肝炎患者乙型肝炎病毒表面大蛋白水平的检测及临床意义  

Detection of HBV large surface protein level and its clinical significance in patients with hepatitis B

作　　者：金霆[1] 

机构地区：[1]福建省福州市第一医院检验科,350013

出　　处：《检验医学与临床》2008年第24期1479-1480,共2页Laboratory Medicine and Clinic

摘　　要：目的探讨乙型肝炎病毒e抗原(HBeAg)阴性慢性乙型肝炎(下称乙肝)患者乙肝病毒表面大蛋白(LHBs)水平变化及其临床意义。方法采用酶联免疫吸附试验(ELISA)法对807例HBeAg阴性血清标本进行LHBs检测,全自动生化分析仪检测丙氨酸氨基转移酶(ALT)。结果(1)在807例HBeAg阴性慢性乙肝患者血清中乙肝病毒DNA阳性率为54.40%(439/807),LHBs的阳性率为56.75%(458/807),二者比较差异无统计学意义(χ2=0.813,P>0.05),二者总体符合率为91.70%(740/807);(2)LHBs含量与乙肝病毒DNA拷贝数呈正相关(r=0.995,P<0.001);(3)LHBs含量与ALT呈正相关(P<0.05)。结论LHBs与乙肝病毒DNA具有良好的相关关系,能够反映出HBeAg阴性乙肝患者体内乙肝病毒复制的程度和疾病的活动状态。Objective To explore the HBV large surface protein （LHBs） level and its clinical significance in patients with HBeAg-negative chronic hepatitis B. Methods Enzyme linked immunosorbent assay （ELISA） method was used to examine the LHBs and the automated biochemistry analyzer was used to detect the level of alanine aminotransferase （ALT） in 807 cases of HBsAg positive serum samples. Results No significant difference of positive rate was observed between HBV DNA [54.40% （439/807）]and LHBs [56.75%（458/807）] （X^2 =0. 813,P〉0.05） in 807 HBeAg-negative serum samples from patients with chronic hepatitis B, and the total coincidence rate was 91.70% （740/807）; serum LHBs level was positively correlated with serum HBV DNA copies （r= 0. 995, P〈 0. 001） and serum ALT level （P〈0. 05）. Conclusion Our results demonstrats that LHBs level is closely correlated with serum HBV DNA copies, contributing to reflecting the active state of HBV DNA replication and chronic hepatitis B patient＇s condition.

关 键 词：乙型肝炎病毒E抗原 表面大蛋白 丙氨酸氨基转移酶 

分 类 号：R446.1[医药卫生—诊断学] R512.62[医药卫生—临床医学]