院内传播耐甲氧西林葡萄球菌的DNA指纹图谱分析  

Analysis on DNA fingerprint of nosocomial transmitting methicillin-resistent Staphylococci

作　　者：施瑜[1] 王震[1] 刘智成[2] 

机构地区：[1]江苏省镇江市第二人民医院检验科,212002 [2]江苏省镇江市第二人民医院感染管理科,212002

出　　处：《检验医学与临床》2008年第24期1487-1488,共2页Laboratory Medicine and Clinic

摘　　要：目的对医院内传播的12株耐甲氧西林葡萄球菌(MRS)作DNA指纹图谱分析,寻找传播途径,控制院内感染。方法纸片琼脂扩散(K-B)法检测细菌耐药性,聚合酶链反应(PCR)法检测mecA基因携带情况,采用ERIC-PCR作DNA指纹图谱分析。结果12株分离菌耐药表型和基因型相同,均对万古霉素和替考拉宁敏感,对其他13种抗菌药物耐药,mecA基因全部阳性。12株不同来源菌株的DNA同源性达100%。患者家属及医护人员的手和病房物体表面被污染情况严重。结论MRS对抗菌药物呈多重耐药性。患者家属及医护人员手部卫生意识薄弱,这是引起MRS医院内传播的主要原因。应加强监控,防止MRS的医院内感染。Objective To analyze DNA fingerprint of 12 strains of nosocomial transmitting methicillin-resistent Staphylococci, so as to seek for the route of transmission and control hospital onset of infection. Methods The susceptibility of 13 antibiotics was determined by K-B method. The mecA gene was detected with PCR. DNA fingerprint was constructed by ERIC-PCR （enterobacter repetitive intergenic consensus-PCR）. Results The sensitive rate of MRS to vancomycin and teicoplanin was 100% and the resistant rate of MRS to other 13 antibiotics was 100%. All showed mecA gene positive by PCR. The DNA homology of 12 strains of MRS was 100%. MRS was cultivated on the surface of objects and on the hands of medical staffs and family members of patients in the ward. Conclusion MRS shows multidrug resistance. The study indicates that medical personnel and family members of patients pay insufficient attention to hand hygiene. This is the material cause for nosocomial transmission of MRS. We must pay more attention to control of nosoeomial MRS infection.

关 键 词：医院内感染 耐甲氧西林葡萄球菌 耐药性 同源性 

分 类 号：R37[医药卫生—病原生物学] R89[医药卫生—基础医学]