荷叶碱的电化学行为研究及分析应用  

Study on the Electrochemical Behavior of Nuciferine and Its Application

作　　者：许雪琴[1] 李志明[1] 王伟[1] 陈国南[1] 

机构地区：[1]福州大学化学化工学院食品安全分析与检测技术教育部重点实验室,福建福州350108

出　　处：《分析科学学报》2008年第6期645-648,共4页Journal of Analytical Science

基　　金：国家自然科学基金(No.2007350002);福建省自然科学基金(No.2007J0130)

摘　　要：本文详细研究了荷叶碱在玻碳电极上的电化学氧化行为。在pH=5.5的B-R缓冲溶液中，用微分脉冲伏安法测定，荷叶碱于＋0.87V（vs.SCE）左右产生一个灵敏的氧化峰，峰电流与荷叶碱的浓度在0.25-30.0μg／mL的范围内呈良好线性关系，检出限为0.05μg／mL。该方法用于荷叶中总生物碱含量的测定，平均回收率为91.5％。此外对荷叶碱的电化学氧化机理进行了探讨。The electrochemical behavior of nuciferine at the glassy carbon electrode （GCE） has been studied. In the Britton-Robinson buffer （pH=5.5）, nuciferine produced an oxidation peak at ＋870 mV （Vs. SCE）. The oxidation peak current showed a well linear relationship with the concentration of nuciferine in the range of 0.25-30.0μg/mL by differential pulse voltammetry. The detection limit was 0.05μg/mL. The proposed method has been applied to the determination of the total alkaloids in the leaves of Lelumbo nucifera Gaertn, and the average recovery was 91.5%. The electrochemical oxidation reaction mechanism of nuciferine was also investigated.

关 键 词：荷叶碱 微分脉冲伏安法 荷叶 机理 

分 类 号：O657.15[理学—分析化学]