流动注射化学发光法测定黄藤素  

Determination of Palmatine by Flow Injection Chemiluminescence

作　　者：刘丽珍[1] 赵守苗[1] 赵鹏祥[1] 韩素琴[1] 

机构地区：[1]山西师范大学化学与材料科学学院,山西临汾041004

出　　处：《分析科学学报》2008年第6期693-696,共4页Journal of Analytical Science

基　　金：山西省自然科学基金(No.20051022)

摘　　要：碱性条件下，铁氰化钾氧化鲁米诺产生化学发光，黄藤素对该体系的化学发光信号具有显著的增强作用。基于此，建立了一种直接测定黄藤素的流动注射化学发光分析新方法。该方法的线性范围为6.0×10-10－3.0×10^-8mol／L（r=0.9956）和3.0×10^-8－3.0×10^-6mol／L（r=0.9981），检出限为2.5×10^-10mol／L（3σ）。对1.0×10^-9mol／L和1.0×10^-7mol／L的黄藤素连续平行测定11次，其相对标准偏差分别为3.5％和0.73％。该方法用于药物和体液中黄藤素的分析，回收率在96％-102％之间。A novel flow injection chemiluminescence method was developed for the determination of palmatine, based on the strong enhanced effect of palmatine on the chemiluminescence reaction of potassium ferricyanide and luminol in basic medium. The experimental results indicated that, under optimum conditions, the chemiluminescence intensity was linearly related to the concentration of palmatine in the range 6.0×10-10-3.0×10^-8mol/L（r=0.9956） and 3.0×10^-8-3.0×10^-6mol/L（r=0.9981） with a detection limit of 2.5×10^-10mol/L（3σ）. The relative standard deviation was 0.73% and 3.5% for 1.0×10^-9 and 1.0×10^-7 mol/L palmatine （n= 11）, respectively. The method was firstly applied to the determination of palmatine in pharmaceutical preparation and biologic fluids, and has the advantages of high sensitivity, fast response and ease of operation.

关 键 词：化学发光 流动注射 黄藤素 鲁米诺-铁氰化钾-亚铁氰化钾 

分 类 号：O657.39[理学—分析化学]