A novel method for the spectrophotometric determination of cefradine by using sodium nitroprusside as chromogenic reagent  

作　　者：Hua Zhang Ling Li Wu Quan Min Li Xin Zhen Du 

机构地区：[1]College of Chemistry and Environmental Science, Henan Normal University, Xinxiang 453007, China [2]College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, China [3]Henan Radio and Television University, Zhengzhou 453008, China

出　　处：《Chinese Chemical Letters》2008年第12期1470-1474,共5页中国化学快报（英文版）

摘　　要：A novel method is developed for the determination of cefradine by using sodium nitroprusside as chromogenic reagent. The experiment indicates that a russety product is formed by the reaction of cefradine with sodium nitroprusside in basic solution, and the maximum absorption wavelength （λmax） of russety product is 505 rim. And the sensitization of tetradecyl benzyl dimethyl ammonium chloride for the reaction of cefradine with sodium nitroprusside is remarkable, The apparent molar absorption coefficient （5505） is 2.81 × 103 L/mol cm. The linear equation isA = 0.0657 ＋ 0.00804C （μg/mL） in the range of 1.50-55.0μg/mL of cefradine with a correlation coefficient r = 0.9992, and the detection limit is 1.38 p,g/mL. This method has been applied to determine cefradine in capsule and tablet samples.A novel method is developed for the determination of cefradine by using sodium nitroprusside as chromogenic reagent. The experiment indicates that a russety product is formed by the reaction of cefradine with sodium nitroprusside in basic solution, and the maximum absorption wavelength （λmax） of russety product is 505 rim. And the sensitization of tetradecyl benzyl dimethyl ammonium chloride for the reaction of cefradine with sodium nitroprusside is remarkable, The apparent molar absorption coefficient （5505） is 2.81 × 103 L/mol cm. The linear equation isA = 0.0657 ＋ 0.00804C （μg/mL） in the range of 1.50-55.0μg/mL of cefradine with a correlation coefficient r = 0.9992, and the detection limit is 1.38 p,g/mL. This method has been applied to determine cefradine in capsule and tablet samples.

关 键 词：CEFRADINE Sodium nitroprusside Chromogenic reagent SPECTROPHOTOMETRY 

分 类 号：TQ2[化学工程—有机化工]