紫杉醇对骨髓细胞体外诱导分化的巨噬细胞影响  

Effects of paclitaxel on the macrophages derived from bone marrow cells in vitro

作　　者：李龙[1,2] 沈红[1,2] 陈静[1,2] 赵勇[2] 

机构地区：[1]北京农学院动物科学技术系,北京102206 [2]中国科学院动物研究所生物膜与膜生物工程国家重点实验室,北京100101

出　　处：《细胞与分子免疫学杂志》2008年第12期1137-1139,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金重点项目(30630060);北京市兽医学(中医药)重点实验室开放课题(200803)

摘　　要：目的:研究紫杉醇对小鼠骨髓分化巨噬细胞的直接影响。方法:常规方法无菌制备BALB/c小鼠骨髓细胞,用含M-CSF的RPMI1640培养液培养7d,同时加入不同浓度紫杉醇,通过流式细胞术对骨髓单核细胞分化的巨噬细胞的表型分子、吞噬功能进行测定,采用迟发型过敏反应(DTH)方法检测巨噬细胞免疫原性。结果:紫杉醇明显降低骨髓单核细胞分化成巨噬细胞的数量;F4/80+巨噬细胞表面分子CD80、CD14表达升高,而I-Ad表达降低;紫杉醇提高分化的巨噬细胞吞噬鸡红细胞的能力;但使其免疫原性降低。结论:紫杉醇能使骨髓单核细胞分化的巨噬细胞细胞吞噬能力提高,但其免疫原性下降,提示紫杉醇可能具有调节巨噬细胞免疫功能的作用。AIM： To Study on the effects of paclitaxel on macrophages derived from the mouse bone marrow cells. METHODS： The bone marrow cells flushed from BALB/c tibia, femur and hipbone were resuspended in RPMI1640 containing M-CSF and seeded in six-well plates. Then they were cultured in RPMI1640 containing M-CSF in the presence or absence of paclitaxel for seven days. The phenotypes and phagocytosis of the macrophages derived from mice bone marrow cells were measured by FCM and their antigen presentation was detected by DTH. RESULTS： The expression of CD80 and CD14 in paclitaxal-treated macrophages was increased and their function of phagocytosis was significantly enhanced. More reduction appeared in DTH reaction of the macrophages treated with palitaxal compared with that in the macrophages derived from control group. CONCLUSION： Paclitaxel can signifi-cantly enhance the phagocytosis of the macrophages derived from bone marrow cells and decrease their immunoge-nicity, which indicates that paclitaxal may regulate the immunological function of the macrophages derived from bone marrow cells.

关 键 词：紫杉醇 巨噬细胞 吞噬 

分 类 号：R392.11[医药卫生—免疫学]