CFSE标记抗原特异性CD4^+CD25^+T细胞在胰岛移植体内归巢的研究  

Homing of CFSE-labeled antigen-specific CD4^+CD25^+ regulatory T cells in islets transplantation

作　　者：张梅[1] 徐书杭[1] 徐瑜[1] 刘翠萍[1] 茅晓东[1] 徐宽枫[1] 刘超[1] 

机构地区：[1]南京医科大学第一附属医院内分泌科,江苏南京210029

出　　处：《细胞与分子免疫学杂志》2008年第12期1174-1176,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：江苏省政府医学重点学科基金项目资助(2001-34)

摘　　要：目的:观察抗原特异性CD4+CD25+Treg细胞在小鼠同种异体胰岛移植后体内的分布特点,探讨抗原特异性CD4+CD25+Treg细胞免疫调节可能机制。方法:构建小鼠同种异体胰岛移植模型。CFSE对抗原特异性CD4+CD25+Treg细胞标记,经尾静脉输注。用组织冰冻切片和流式细胞术动态了解CFSE标记的抗原特异性CD4+CD25+Treg细胞在受体内的分布和增殖变化。结果:抗原特异性CD4+CD25+Treg细胞联合胰岛移植组胰岛移植物平均生存期为(34.57±17.15)d,较胰岛移植组生存时间(10.6±1.82)d,差异有统计学意义(P<0.01)。抗原特异性CD4+CD25+Treg细胞在各级淋巴结均有能定居,但是胰腺淋巴结定居的细胞数量明显多于其他淋巴结。结论:抗原特异性Treg细胞抑制STZ诱导的糖尿病小鼠的急性移植排斥反应,细胞抑制功能与细胞在体内淋巴结的归巢有关。AIM： To observe the distribution and prolif- eration of the antigen-specific CD4^＋ CD25^＋ regulatory T （Treg） cells and investigate the potential mechanism of antigen-specific CD4^＋ CD25^＋ regulatory T cells on the suppression of rejection for allogenetic islet transplantation in vivo. METHODS： Antigen-specific CD4^＋ CD25^＋ Treg cells were generated by the addition of multiple intravenous injections of ICR mice splenocytes in vivo. After labeled by CFSE, 8×10^5 antigen-specific Treg cells were injected via tail vein with islets transplantation. Homing and distribution of antigen-specific CD4^＋ CD25^＋ Treg cells were investigated by flow cytometric analysis and immunofluorescence. RESULTS： In vivo, the mean survival time of recipients with islets and antigen-specific CD4^＋ CD25^＋ Treg cells were （34.57±17.15） days, whereas transplanted islets without Treg treatment survived （10.6±1.82） days in control mice. The transferred antigen-specific CD4^＋ CD25^＋ Treg cells were mainly resident in pancreatic node and mesenteric node. CONCLUSION： Allogeneic antigen-specific CD4^＋ CD25^＋ Treg cells prolong islet graft survival, and draining lymphoid tissue play a key role in the immune response.

关 键 词：CD4^+CD25^+ 调节性T细胞 胰岛/移植 CFSE 

分 类 号：R392.11[医药卫生—免疫学]