维甲酸诱导小鼠胚胎干细胞向神经样干细胞分化  

Embryonic Stem Cells Following Retinoic Acid Administration Differentiated into Neuronal-like Stem Cells in Vitro

作　　者：杨慧娟[1] 黄桂琴[1] 李琴[1] 王廷华[1] 

机构地区：[1]昆明医学院神经科学研究所,云南昆明650031

出　　处：《四川解剖学杂志》2008年第4期24-26,共3页Sichuan Journal of Anatomy

摘　　要：目的探讨维甲酸(RA)诱导体外培养的小鼠胚胎干细胞(ESC)向神经样干细胞定向分化。方法在无白血病抑制因子(LIF)存在的条件下,将体外培养的ESC悬浮培养4天,形成拟胚体(EB),再经RA诱导4天后进行细胞冰冻切片,行免疫细胞化学染色计数生殖特异性基因Oct-4和神经干细胞特异性标志物Nestin的表达百分率。结果经诱导的ESC呈现Oct-4阳性细胞百分率为67.34%,而Nestin阳性细胞百分率为36.52%。结论体外培养的小鼠ESC经RA诱导后有部分细胞定向诱导分化为神经样干细胞。Objective To investigate embryonic stem cells （ESC） cultured in vitro following retinoic acid （RA） administration differentiated into neuronal-like stem cells. Methods ESC were cultured 4 days in vitro without leukemia inhibitory factor（LIF） and RA, then administrated by RA in following 4 days of culture. This was followed by made for frozen sections of ESC for Oct-4 and Nestin immunocytochemical staining. Results The Oct-4 immunopositive ESC percentage was 67. 34% and Nestin immunopositive derived from ESC percentage was 36.52%. Conclusion ESC induced by RA were partly differentiated into neuronal-like stem cells.

关 键 词：维甲酸 胚胎干细胞 神经样干细胞 免疫细胞化学 OCT-4 NESTIN 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]