割胶、机械伤害和外源茉莉酸对橡胶树乳管细胞防卫蛋白基因表达的影响  

Effects of Tapping,Mechanical Wounding and Exogenous Jasmonates on the Expression of Genes Encoding for Defense Proteins in the Latex of Hevea brasiliensis

作　　者：杨署光[1] 于俊红[2] 史敏晶[1] 陈月异[1] 田维敏[1] 

机构地区：[1]中国热带农业科学院橡胶研究所农业部热带作物栽培生理学重点开放实验室,海南儋州571737 [2]广东省农业科学院土壤肥料研究所,广州510610

出　　处：《热带作物学报》2008年第5期535-540,共6页Chinese Journal of Tropical Crops

基　　金：973前期研究专项(编号:2006CB708205);国家自然科学基金项目(编号:30671712);中国热带农业科学院科技基金项目(编号:Rky0501)资助

摘　　要：为了进一步探明巴西橡胶树乳管的防卫功能,笔者采用RT-PCR和Northern-blot技术分析割胶、机械伤害和外源茉莉酸对胶乳几丁质酶、β-1,3-葡聚糖酶和橡胶素基因表达的影响,结果表明:(1)外源茉莉酸和机械伤害均能上调几丁质酶、β-1,3-葡聚糖酶和橡胶素的基因表达,但机械伤害的效应滞后于外源茉莉酸;(2)几丁质酶、β-1,3-葡聚糖酶和橡胶素的基因在割胶树中的表达明显强于在未开割树中的表达;(3)割胶树胶乳中内源茉莉酸含量显著高于未开割树。There are three defense proteins, i.e., chitinase, β-1,3-glucanase and hevein, in the lutoids of latex from rubber tree. These proteins may be accumulated around the laticifer cut to form a biochemical barrier against pathogenic entrance after being released from lutoids which are usually broken during latex flow. Little is known about the mechanism for the regulation of expression of genes encoding for these proteins. The expression of these genes induced by tapping, mechanical wounding and exogenous jasmonates was analyzed by RT-PCR and Northern blot. The expression of genes encoding for chitinase, β-1,3-glucanase and herein was up-regulated by mechanical wounding and exogenous jasmonates, with the effect of the former being weaker than that of the latter. The expression of these genes is remarkably stronger in trees opened for tapping than in the virgin trees. The level of endogenous jasmonates in the latex is significantly higher in the tapped trees than in the virgin trees. The up-regulation of these genes by mechanical wounding and tapping may be due to the increase in the level of endogenous jasmonates in bark tissues and latex. The increase in the level of endogenous jasmonates in latex caused by tapping may play an important role in enhancing the defense of rubber trees against pathogens.

关 键 词：巴西橡胶树 乙烯 茉莉酸 防卫蛋白 基因表达 

分 类 号：S794.1[农业科学—林木遗传育种]