45S rDNA和5S rDNA在水仙染色体上的物理定位  

Chromosome Localization of 45S rDNA and 5S rDNA in Chinese Narcissus by Fluorescence in situ Hybridization

作　　者：吴菁华[1] 张志忠[1] 吕柳新[1] 

机构地区：[1]福建农林大学园艺学院,福建福州350002

出　　处：《热带作物学报》2008年第5期618-621,共4页Chinese Journal of Tropical Crops

基　　金：福建省自然科学基金计划项目(编号:2007J0245);福建省教育厅资助科技项目(编号:JA07069);国家自然科学基金项目(编号:30771488)资助

摘　　要：利用荧光原位杂交技术对水仙45S rDNA和5S rDNA进行定位分析。结果表明,45S rDNA信号共有2对,分别分布在水仙的第6号染色体和第7号染色体短臂的末端,且第7号染色体上的拷贝数多于第6号染色体的;而5S rDNA则只有1对杂交信号,位于第2号染色体的长臂,信号较弱。With fluorescence in situ hybridization （FISH） , the sites of the 45SrDNA and 5S rDNA on the mitotic metaphase chromosomes of Chinese Narcissus （Narcissus tazeta var. White Flower） were located and analyzed. The results indicated that there were two pairs of signals detected for 45S rDNA. According to the characteristics of the chromosome, the chromosome with bigger signal was No.7 and the other pair was chromosome No.6. The signal of 5S rDNA was relatively weak, and it was located on 2L. These results provide some molecular cytogenetics information for further study of changes of chromosomes in Narcissus evolution.

关 键 词：水仙 荧光原位杂交 RDNA 染色体 

分 类 号：S682.21[农业科学—观赏园艺]