毛细管电泳安培法用于尿样中β-阻断剂类兴奋剂药物的研究  

Separation and Determination of β-Blockers Doping in Urine by Capillary Electrophoresis Amperometric Detection

作　　者：宋子旺[1] 童萍[1] 郑丽辉[1] 张兰[1] 

机构地区：[1]福州大学食品安全分析与检测教育部重点实验室,福州大学运动科学研究中心,福州350002

出　　处：《分析化学》2008年第12期1624-1628,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20675016);福建省体育局重大专项(No.HX2005-74);福建省科技厅重点项目(No.2007I0020,2007Y0060);福建省自然科学基金资助项目(No.B0510006,2007J0219);福建省高校新世纪优秀人才支持计划(No.HX2006-101)资助项目

摘　　要：利用毛细管电泳-安培法检测联用技术，建立了一种简单、灵敏、快速同时分离检测人体尿液中两种β-阻断剂类兴奋剂药物索他洛尔和塞利洛尔的新方法。以直径300μm的铂圆盘电极为检测电极（铂丝为辅助电极，Ag／AgCl为参比电极），在0.950V的检测电位下，30mmol／L Clark-Lubs缓冲体系（pH9．60）中，两种分析物在8min内达到基线分离。在最佳条件下，其峰电流与0.15～60mg／L索他洛尔与0．30～60mg／L塞利洛尔呈现良好的线性关系，检出限分别为0．05mg／L和0．09mg／L，并将建立的方法成功地应用于人体尿样的分析。进～步研究了人体尿液中索他洛尔36h内的排泄曲线及索他洛尔在尿液中的排泄规律，对奥运会中的兴奋剂快速检测具有一定的应用价值。A simple, sensitive, and quick method was established to separate and determine two β-blockers, sotalol and celiprolol in human urine by capillary electrophoresis-amperometric detection. Under optimal conditions of the detection potential of 300 μm platinum disk electrode at O. 950 V （vs. Ag/AgCl）, pH 9.60, 30 mmol/L Clark-Lubs buffer solution, the two analytes were baseline separated in 8 min. The calibration curves exhibited an excellent linear behavior over the concentration range about three orders of magnitude. The detection limits were 0.05 and 0.09 mg/L for sotalol and celiprolol, respectively. The method was successfully applied to the detection of sotalol in the human urine after 36 h of administration of sotalol tablets, and the metabolism curve of it was also obtained.

关 键 词：毛细管电泳 安培法检测 Β-阻断剂 兴奋剂 索他洛尔 塞利洛尔 

分 类 号：R87[医药卫生—运动医学]