热解吸-气相色谱法测定植物源挥发性有机物  

Analysis of Biogenic Volatile Organic Compounds by Thermal Desorption and Gas Chromatography

作　　者：李德文[1,2] 何兴元[1] 史奕[1] 

机构地区：[1]中国科学院沈阳应用生态研究所,沈阳110016 [2]中国科学院研究生院,北京100039

出　　处：《分析化学》2008年第12期1690-1694,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.90411019)资助项目

摘　　要：以Tenax-TA、Carboxen 1000和Carbosieve SⅢ为采样管填充料,将植物源挥发性有机物吸附于采样管内,样品通过二次热解吸仪解吸后,随载气进入气相色谱仪,采用氢火焰离子化检测器(FID)测定,建立了利用二次热解吸仪与气相色谱联用技术测定植物挥发性有机物的分析方法。载气N2流速为30 mL/min,60℃下吹扫吸附管2 min,然后在250℃下解吸吸附管5 min,冷却1 min后,在275℃下解吸聚焦管3 min,样品经传输线进入气相色谱。气相色谱载气N2压力为190 kPa,FID检测器温度280℃;进样口温度225℃;初始柱温40℃,停留5 min,以2℃/min升温至120℃,保留1 min,然后以20℃/min升温至200℃,保留10 min。方法重现性好,精密度高,线性相关系数大于0.99;检出限均低于9×10-9g/L;解吸效率大于96%,适用于植物源挥发性有机物的测定。A thermal desorption and gas chromatographic method for the analysis of biogenic volatile organic compounds （BVOCs） was established. The air samples were collected on glass adsorbent tubes filled with Tenax-TA, Carboxen 1000 and Carbosieve SⅢ. The sample was thermal desorbed, and then determined by gas chromatography with flame ionization detector. The pre-concentrated samples were dry at 60℃ for 2 min, and then thermally desorbed at 250 ℃ for 5 rain at 30 mL/min, and secondary desorption was at 275 ℃ for 3 min. A GC-FID was used to detect and. quantify BVOCs. An initial oven temperature of 40 ℃ was maintained for 5 min, and then increased to 120 ℃ at 2 ℃/min for 1 rain, followed by an increase at 20 ℃/min to 200 ℃ for 10 min. The results showed that eight kinds of BVOCs could be separated by the gas chromatographic condition, which had good precisions and high recoveries. The objective compounds can be calculated very accuracy by a standard curve individually （average r above 0.99）. The precision is lower than 5% and the minimum detectable quantity was 4 × 10^-10 g/L. The desorption efficiency was higher than 96%. The method provides a new way for intensifying the BVOCs.

关 键 词：热解吸 气相色谱 植物源挥发性有机物 

分 类 号：Q946[生物学—植物学]