硝普钠显色测定药物样品中甲巯咪唑  

Determination of Thiamazole in Pharmaceutical Samples Using Sodium Nitroprusside as the Chromogenic Agent

作　　者：王敏[1] 吴伶俐 李全民[1] 

机构地区：[1]河南师范大学化学与环境科学学院,河南省环境污染控制重点实验室,新乡453007 [2]河南广播大学农医学院,郑州450008

出　　处：《分析化学》2008年第12期1725-1728,共4页Chinese Journal of Analytical Chemistry

基　　金：河南省教育厅自然科学基金(No.2008B150011)资助项目

摘　　要：在溶液中NaOH浓度为6．7×10^-3mol／L及0℃（冰水浴）条件下，反应时间为30min，硝普钠与甲巯咪唑生成化学计量比为1碱性条件下硝普钠与甲巯咪唑反应生成化学计量比为1：1的绿色产物，其最大吸收波长为610nm。甲巯咪唑在4．8～48mg／L浓度范围内与吸光度呈良好线性关系，线性回归方程为A=-0．0617＋0．01329C（mg／L），相关系数r=0．9991；检出限为0．47mg／L；相对标准偏差RSD=0．2％。本法成功用于测定片剂药品中甲巯咪唑的含量，平均回收率大于97．2％。The experiment indicated that a green product could be formed by the reaction of thiamazole and sodium ntroprusside, when NaOH was 6.7 × 10^-3 mol/L in the solution, and temperacture and reaction time were 0℃ and 30 min, respectively. The stoichimetrie ratio of the green product was 1：1. The maximal absorption wavelength of the product was 610 nm, ε610 = 1.5 × 10^3L/（mol·cm）. Good linear relationship was obtained between the absorbance and the concentration of thiamazole in the range of 4.8-48 mg/L. The equation of the linear regression was A = -0. 0617 ＋ 0.01329C（mg/L） , with a linear correlation coefficient of 0.9991. The detection limit was 0.47 rag/L, and the relative standard deviation was 0. 2%. The method has been successfully applied to the determination of thiamazole in tablets with a average recovery of 97.2%.

关 键 词：硝普钠 甲巯咪唑 分光光度 

分 类 号：R927[医药卫生—药学]