细脚拟青霉多糖PTPS-1的结构特征和抗肿瘤活性的初步研究  

Structural characters and antitumor activity of a polysaccharide PTPS-1 from Paecilomyces tenuipes

作　　者：金丽琴[1] 左江成[2] 吕建新[2] 李宝剑[2] 李东[2] 

机构地区：[1]温州医学院临床医学部生物化学教研室,浙江温州325035 [2]温州医学院细胞与分子医学研究所,浙江温州325035

出　　处：《中草药》2008年第12期1772-1776,共5页Chinese Traditional and Herbal Drugs

基　　金：浙江省自然科学基金资助项目(301036)

摘　　要：目的分析鉴定细脚拟青霉多糖PTPS-1的理化性质和结构特征,并观察其抗肿瘤活性。方法采用DEAE-纤维素及Sephadex G-100柱色谱分离纯化得到PTPS-1;利用HPLC、GC、IR、部分酸水解及NMR分析PTPS-1的相对分子质量、组成和结构特征;采用CCK-8检测观察PTPS-1对小鼠脾细胞和PTPS-1刺激培养的小鼠腹腔巨噬细胞上清对Sp2/0细胞的增殖活性。结果PTPS-1的纯度为98.977%,平均相对分子质量为1.84×104,由甘露糖和半乳糖组成,二者摩尔比为1.3∶1,糖链结构中主链含有α-(1→6)、α-(1→2)和β-(1→3)连接的糖苷键,支链含有Gal-(1→3)连接。PTPS-1可明显促进小鼠脾细胞的增殖(P<0.01),PTPS-1刺激培养的小鼠腹腔巨噬细胞上清能抑制Sp2/0细胞的增殖(P<0.01)。结论PTPS-1是从细脚拟青霉中分离出的具有免疫调节和抗肿瘤活性的中性杂多糖。Objective To investigate the physicochemical properties, structural characters and antitumor activity of a polysaccharide PTPS-1 from Paecilornyces tenuipes. Methods PTPS-1 was obtained by DEAE-cellulose and Sephadex G-100 chromatography column. The methods of HPLC, GC, IR, and NMR were used to investigate the molecular weight, monosaccharide composition, and structural characters of PTPS-1. The CCK-8 kit was used to determine the proliferation of PTPS-1 on murine splenocyte and the supernatant of the murine macrophage with PTPS-l-stimulated on Sp2/0 cells. Results PTPS-1, whose molecular weight was 1.84 × 10^4 and purity was 98. 977%, contained mannose （Man） and galactogen （Gal） as monosaccharide constitute in molar ratio of 1.3 ： 1. It consisted of a backbone of α-（1→6）, α-（1 →2）, and β-（1→3） linkage, whose branches were made by Gal-（1→3） linkage. PTPS-1 significantly stimulated the murine splenocyte proliferation and the supernatant of macrophage inhibited the growth of Sp2/0 cells in vitro. Conclusion PTPS-1 is a polysaccharide from P. tenuipes with immunomodulatory and antitumor activity.

关 键 词：细脚拟青霉 多糖类 抗肿瘤活性 

分 类 号：R284.2[医药卫生—中药学]