丹皮与关木通配伍对马兜铃酸Ⅰ的影响  

Effect of compatibility between Caulis Aristolochiae Manshuriensis and Cortex Moutan on aristolochic acid Ⅰ

作　　者：王勇[1] 邓晓春[1] 

机构地区：[1]深圳大学生命科学学院深圳市微生物基因工程重点实验室,广东深圳518060

出　　处：《中草药》2008年第12期1805-1807,共3页Chinese Traditional and Herbal Drugs

基　　金：深圳市科技计划资助项目(200528)

摘　　要：目的研究马兜铃酸的热稳定性和配伍对关木通煎煮液中马兜铃酸Ⅰ的影响,探讨马兜铃酸Ⅰ的配伍减毒机制。方法HPLC法测定关木通单煎液、关木通与丹皮共煎液、马兜铃酸Ⅰ对照品单煎液、关木通单煎后药渣和关木通与丹皮共煎后的关木通药渣中的马兜铃酸Ⅰ的量。结果马兜铃酸Ⅰ在纯水中煎煮后减少,并在其保留时间前又出现一个色谱峰,此峰同样出现在关木通煎煮液中。丹皮与关木通药对共煎液中的马兜铃酸Ⅰ的量低于关木通单煎液,并且共煎后的关木通残渣中的马兜铃酸Ⅰ也低于单煎后残渣。结论马兜铃酸Ⅰ在加热的条件下不稳定,部分生成另外一种物质。丹皮与关木通配伍后没有抑制关木通中马兜铃酸Ⅰ的溶出量,配伍减少关木通煎煮液中马兜铃酸Ⅰ的原因是后者溶出后发生了化学变化。Objective To investigate the thermostability of aristolochic acid Ⅰ and the effect of the compatibility on aristolochic acid Ⅰ in Caulis Aristolochiae Manshuriensis decoction and to explore the detoxification mechanism of compatibility for aristolochic acid Ⅰ . Methods Analyzing the contents of aristolochic acid Ⅰ by HPLC in the single decoction of Caulis Aristolochiae Manshuriensis, the concoction of Caulis Aristolochiae Manshuriensis with Cortex Moutan, the residues of decocted Caulis Aristolochiae Manshuriensis and the residues of concocted Caulis Aristolochiae Manshuriensis with Cortex Moutan, respectively. Results Aristolochic acid Ⅰ decreased after heating in pure water, a new peak was found in HPLC spectra and supposed to be the derivate of aristolochic acid Ⅰ , which was also found in the decoction of Caulis Aristolochiae Manshuriensis. The content of aristolochic acid Ⅰ in the concoction of Cortex Moutan with Caulis Aristolochiae Manshuriensis is lower than that in the single Caulis Aristolochiae Manshuriensis decoction. Furthermore, the quantity of aristolochic acid Ⅰ in the residues of Caulis Aristolochiae Manshuriensis after concoction is lower than that in the residues of single Caulis Aristolochiae Manshuriensis decoction. Conclusion Aristolochic acid Ⅰ is unstable in decoction and a part of it was changed into another compound. The stripping of aristolochic acid Ⅰ from Caulis Aristolochiae Manshuriensis is not inhibited when Cortex Moutan concocted with Caulis Aristolochiae Manshuriensis. It is the chemical reaction of aristolochic acid Ⅰ , which could decrease the toxicity in the decoction or concoction of Caulis Aristolochiae Manshuriensis.

关 键 词：关木通 丹皮 配伍 马兜铃酸Ⅰ 

分 类 号：R286.1[医药卫生—中药学]