20(S)-原人参二醇对肺癌A549细胞增殖和荷瘤裸小鼠肿瘤生长的抑制作用  

Inhibition of 20(S)-protopanaxadiol on proliferation of lung cancer A549 cells and tumor growth of nude mice

作　　者：张锐[1] 徐华丽[1] 曲绍春[1] 于小风[1] 陈明侠[1] 睢大赟[1] 

机构地区：[1]吉林大学药学院药理教研室,吉林长春130021

出　　处：《中草药》2008年第12期1838-1841,共4页Chinese Traditional and Herbal Drugs

基　　金：国家"九五"重点科技攻关课题(96-901-01-83)

摘　　要：目的研究20(S)-原人参二醇对小细胞肺癌A549细胞增殖和荷瘤裸小鼠肿瘤生长抑制作用。方法采用噻唑蓝(MTT)法观察20(S)-原人参二醇对小细胞肺癌A549细胞增殖的抑制作用,流式细胞术测定20(S)-原人参二醇对小细胞肺癌A549细胞凋亡及周期的影响,并在裸小鼠人肺癌模型上观察20(S)-原人参二醇对荷瘤裸小鼠肿瘤生长的抑制作用。结果20(S)-原人参二醇对A549细胞具有明显的抑制作用并有剂量时间依赖关系,并且流式细胞仪检测时均出现典型凋亡峰,24小时凋亡率分别为32.47%、32.75%、33.51%。荷瘤裸小鼠动物实验表明,20(S)-原人参二醇对裸小鼠的肿瘤生长也具有明显的抑制作用,抑瘤率分别为18.78%、34.37%、50.02%。结论20(S)-原人参二醇对A549细胞的增殖和荷瘤裸小鼠肿瘤的生长具有明显的抑制作用。Objective To study the effects of 20 （S ）-protopanaxadiol （PPD） on the proliferation of human lung carcinoma A549 cell line and the tumor growth of nude mice. Methods Human lung carcinoma A549 cell line was treated with PPD. Cell growth rate was determined with MTT assay. The effects of PPD on apoptosis rate and cell cycle were observed by the flow cytometer. The subcutaneous transplantable tumor model of human lung cancer in nude mice was established. The tumor inhibitory rate was calculated to evaluate the anti-tumor effect. Results PPD could inhibit the proliferation of human lung carcinoma A549 cell in a time- and dose-dependent manner. The apoptotic peak was observed in the PPD groups with the apoptosis rate of 32.47%, 32.75%, and 33.51% in 24 h, respectively. And PPD inhibited the growth of human lung carcinoma A549 cells in nude mice with the tumor inhibitory rate of 18. 78%, 34.37%, and 50.02%. Conclusion 20 （S）-Protopanaxadiol could inhibit the proliferation of lung cancer A549 cell and the tumor growth of nude mice.

关 键 词：20(S)-原人参二醇 A549细胞 肺癌 裸小鼠 

分 类 号：R286.91[医药卫生—中药学]