巴戟天遗传多样性的RAPD研究  

Genetic diversity of Morinda officinalis by RAPD

作　　者：丁平[1] 刘瑾[1] 仰铁锤[1] 邱金英[1] 

机构地区：[1]广州中医药大学中药学院,广东广州510405

出　　处：《中草药》2008年第12期1869-1872,共4页Chinese Traditional and Herbal Drugs

基　　金：广东省自然科学基金项目(04010057);重点项目(06105061);国家科技支撑计划子课题(2006BAI06A11-02)

摘　　要：目的应用随机扩增多态性DNA(random amplified polymorphic DNA,RAPD)标记技术对广东产5个类群的栽培巴戟天进行遗传多样性研究。方法应用15种引物对64个个体进行检测,应用Popgen32软件分析群体的遗传多样性。结果共得到224条带,其中多态位点112个,多态百分率为50.00%。种群内多态位点百分率在37.05%～53.13%,平均为45.86%。Shannon指数和Nei指数均反映出巴戟天各类群遗传多样性有一定的变化。Shannon指数为0.287 6,各类群的Shannon指数在0.103 3～0.236 2,Nei指数为0.175 6,各类群的Nei指数在0.074 5～0.154 0。结论目前栽培巴戟天居群间有一定的分化,并产生了多种农家类型,其中小叶巴戟天(POP2)遗传多样性明显高于其他类型,这些可能是导致目前栽培巴戟天质量产生变异的主要原因。Objective To investigate the genetic diversity of five populations of cultivated Morinda officinalis in Guangdong Province using random amplified polymorphic DNA （RAPD）. Methods Sixty- four individuals in five populations of cultivated M. officinalis were analyzed by RAPD markers to determine the genetic variations among the populations. The data of genetic diversity were analyzed with Popgen32 software. Results The levels of genetic variation and patterns of population structure in M. officinalis were investigated using RAPD markers. Of the 100 primers screened, 15 primers produced highly reproducible RAPD bands, using these primers, 224 discernible DNA fragments were generated with 112 （50. 00%） polymorphic fragments, indicating considerable genetic variation at the species level. In contrast, there were relatively high levels of polymorphism at the population level with the percentage of polymorphic bands ranging from 37.05% to 53.13%, and the mean percentage of polymorphic loic （P= 45.86%）. Genetic variation among populations was detected based on Nei＇s genetic diversity analysis （0. 175 6）, Shannon＇s diversity index （0. 287 6） for every population with Shannon＇s index 0. 103 3- 0. 236 2 and Nei＇s indexes 0. 074 5-0. 154 0. Conclusion There is a little genetic differentiation among populations of cultivated M. officinalis and result in different cultivated types, the genetic diversity with M. officinalis （POP2） is higher than that of other populations. It may be the main reason of the difference of cultivated M. officinalis quality.

关 键 词：巴戟天 遗传多样性 RAPD分析 

分 类 号：R282.7[医药卫生—中药学]