不同水分和氮素条件对栽培绞股蓝生物量和皂苷量的影响  

Effect of different water and nitrogen on biomass and gypenosides in Gynostemma pentaphyllum

作　　者：龙云[1] 杨睿[2] 钟章成[1] 谈锋[1] 

机构地区：[1]西南大学生命科学学院,三峡库区生态环境教育部重点实验室,重庆市三峡库区植物生态与资源重点实验室,重庆400715 [2]西南大学化学化工学院,重庆400715

出　　处：《中草药》2008年第12期1872-1876,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30370279,30670334);西南大学科技基金资助项目(2001-Q12)

摘　　要：目的研究人工栽培条件下水分和氮素对绞股蓝Gynostemma pentaphyllum生物量和皂苷量的影响。方法对野生绞股蓝扦插苗进行水分和氮素控制实验。结果在干旱和缺氮逆境下,整株植株生物量减少,但由于β-糖苷酶活性降低,导致单位干质量叶片中绞股蓝皂苷的量增加。由于干旱使生物量减少的速率大于对皂苷积累的促进作用,表现为干旱使总皂苷产量降低,与之相反,缺氮对生物量减少的速率小于对皂苷积累的促进作用,但是氮素浓度过低也不利于皂苷积累。结论这意味着在人工种植的情况下,必须保证充足的水分供应和适度的氮肥供应才有利于绞股蓝生长,保持较高的生物量和总皂苷产量。Objective To investigate the effect of different water and nitrogen on blomass anti gypenosides in Gynostemma pentaphyllurn. Methods The cutting of G. pentaphyllum in Jingyun Mountain of Chongqing was used as materials in the experiments by artificial imposed water stress and various N availability. Results Under soil drought or N-deficit conditions, the biomass of whole plant decreased, while the content of total saponins in leaves on dry weight basis increased, the content of total saponins increased due to the decreased activity of β-glucosidase. Because of the decreasing rate of biomass of G. pentaphyllum was larger than the accumulating rate of total saponins, the total yields of total saponins decreased under water deficit condition. In contrast, the decreasing rate of biomass of G. pentaphyllurn was lower than the accumulating rate of total saponins under N-deficit condition, however this does not mean the accumulation of total saponins can benefit from nitrogen supply with too low concentration. Conclusion Therefore, to obtain the higher biomass accumulation of G. pentaphyllum and more saponins content in plant, nitrogen should be applied appropriately and the sufficient supply of water be beneficial for the cultivation of G. pentaphyllum.

关 键 词：绞股蓝皂苷 野生绞股蓝 栽培条件 生物量 氮肥 水分 人工种植 活性降低 

分 类 号：R285.5[医药卫生—中药学] R284.1[医药卫生—中医学]