中国HIV感染者细胞毒性淋巴细胞与CD4^+CD25^+调节性T细胞相关性研究  

Correlation between CD4^+CD25^+Foxp3^+ regulatory T cells and cytotoxic lymphocytes in HIV infected patients

作　　者：张子宁[1] 姜拥军[1] 刘静[1] 金鑫[1] 孙国权[1] 施万英[1] 张旻[1] 王亚男[1] 韩晓旭[1] 尚红[1] 

机构地区：[1]中国医科大学附属第一医院卫生部艾滋病免疫学重点实验室,沈阳110001

出　　处：《中国免疫学杂志》2008年第12期1125-1129,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金(30600532);国家重点基础研究发展计划973计划(2006CB504206)资助

摘　　要：目的:对中国HIV感染者NK细胞、CD8+T细胞及胞内穿孔素、颗粒酶-B表达与CD4+CD25+Foxp3+调节性T细胞水平的相关性进行研究,探讨调节性T细胞在HIV感染中的作用机制。方法:选取73名HIV/AIDS患者(长期不进展组、无症状HIV组、AIDS组),应用流式细胞仪胞内染色技术检测NK细胞、CD8+T细胞数量及胞内穿孔素、颗粒酶-B表达水平,分析其与CD4+CD25+Foxp3+调节性T细胞水平的相关性。结果:CD4+CD25+Foxp3+调节性T细胞百分率与NK细胞、CD8+T淋巴细胞数量呈明显负相关(P<0.01),与CD8+T细胞内穿孔素、颗粒酶-B表达百分率呈明显正相关(P<0.05),与NK细胞内穿孔素、颗粒酶-B表达水平绝对值负相关(P<0.01),与CD8+T细胞内颗粒酶-B表达绝对值呈明显负相关(P<0.01),与CD8+T细胞内穿孔素表达绝对值无明显相关性(P>0.05)。CD4+CD25+Foxp3+调节性T细胞绝对值与CD8+T细胞内穿孔素、颗粒酶-B表达百分率呈明显负相关(P<0.05)。结论:中国HIV感染者细胞毒性淋巴细胞数量功能的变化与调节性T细胞显著相关,提示高水平的调节性T细胞可能与细胞毒性淋巴细胞耗竭相关,可能为导致疾病进展的原因之一。Objective：To detect the relationship between the frequencies of CD4^＋CD25^＋Foxp3^＋ regulatory T cells and cytotoxic lymphocytes in HIV infected patients in China,in order to study the mechanism of regulatory T cells in HIV infection.Methods：73 untreated HIV-1 infected patients and 16 normal controls were enrolled and the frequencies of CD4^＋CD25^＋Foxp3^＋ regulatory T cells,NK cells,CD8^＋T cells and granzyme-B,perforin expression of cytotoxic lymphocytes were detected by flow-cytometry.Results：The frequency of CD4^＋CD25^＋Foxp3^＋Treg cells was inversely related to those of NK,CD8 cells（NK cell：r=-0.502,P〈0.01;CD8^＋T cell：r=-0.437,P〈0.001）.It was positive correlated with the proportion of granzyme-B,perfor in expression of CD8^＋ T cells but inversely correlated with absolute count of granzyme-B,perforin expression of NK cells and perforin expression of CD8 cells.The absolute count of CD4^＋CD25^＋Foxp3^＋Treg cells was inversely related to the proportion of granzyme-B,perforin expression of CD8 cells.Conclusion：The frequency of CD4^＋CD25^＋Foxp3^＋ regulatory T cells are correlated with the change of the number and perforin,grazyme-B expression of cytotoxic lymphocytes,which indicates the high level of regulatory T cells may attribute to the exhausion of cytotoxic lymphocyes,thus affect the disease progression of HIV infection.

关 键 词：HIV-1 调节性T细胞 细胞毒性淋巴细胞 颗粒酶 穿孔素 

分 类 号：R512.91[医药卫生—内科学]