FQ-PCR和AMPLICOR HCV MONITOR 2.0方法测定HCV病毒载量的比较  

Comparison of HCV RNA value using FQ-PCR and AMPLICOR HCV MONITOR 2.0

作　　者：白旭华 毛玮[2] 潘品良[2] 于海英[2] 蒋岩[2] 

机构地区：[1]新疆兵团中心血站,乌鲁木齐830002 [2]中国疾病预防控制中心性病艾滋病预防控制中心国家艾滋病参比实验室,北京100050

出　　处：《中国艾滋病性病》2008年第6期580-582,共3页Chinese Journal of Aids & STD

基　　金：中美艾滋病防治合作项目

摘　　要：目的探讨荧光定量(FQ-PCR)和AMPLICOR HCV MONITOR 2.0试剂盒检测相同样品中丙型肝炎病毒(HCV)病毒载量值之间的关系。方法收集84份样品,经ELISA检测抗-HCV,再分别使用两种方法测定HCV病毒载量,利用统计分析软件SPSS 15.0对病毒载量Log对数值进行统计分析。结果当HCV病毒载量值大于1000IU/ml时,两种方法检测结果的相关性为0.818(P<0.0001)。结论FQ-PCR和AMPLICOR HCV MONI-TOR 2.0两种方法测定的HCV病毒载量值有较高的相关性。Objective To compare the results of detecting HCV RNA value by using FQ-PCR and AMPLICOR HCV MONITOR 2.0s and explore their correlation. Methods Eighty-four clinical samples were collected and HCV viral load was determined with the above mentioned two methods, and their Log10 transmitted values were analyzed by SPSS 15.0.Results The correlation coefficient between the above two methods was 0. 818 （P〈 0.0001 ）when the results of HCV viral load was more than 1 000IU/ml. Conclusion The results of HCV viral load with the two testing methods are well correlated and highly comparable.

关 键 词：丙型肝炎病毒 病毒载量 逆转录聚合酶链反应 荧光定量聚合酶链反应 

分 类 号：R373.21[医药卫生—病原生物学]