重组盐藻14-3-3蛋白基因的原核表达及分离纯化  

Purification and prokaryotic expression of recombined Dunaliella salina 14-3-3 protein gene

作　　者：王天云[1] 昝玉玺[1] 张俊河[1] 张煜[1] 王俐[1] 郭丽[1] 

机构地区：[1]新乡医学院生物化学与分子生物学教研室,新乡453003

出　　处：《四川大学学报（自然科学版）》2008年第6期1533-1536,共4页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金(30470030)

摘　　要：根据克隆的杜氏盐藻14-3-3蛋白的基因序列设计引物,采用RT-PCR方法扩增杜氏盐藻14-3-3蛋白的基因.经原核表达、Ni2+亲和层析法纯化,免疫动物制备抗体.抗体蛋白质印迹分析检测结果表明抗体能特异结合盐藻细胞分子量为29 kD的多肽.RT-PCR was accomplished to clone 14-3-3 gene was expressed in E. coli BL21 with induction of protein gene from Dunalilla salina. The recombinant IPTG, purified by Ni2 ＋ affinity chromatography. Fu- sion proteins were isoIated and then lyophilized to raise the polyclonal antisera against 14-3-3 respectively by immunizing rabbits. The antibodies could bind to 29 kD polypeptides of D. salina specificity.

关 键 词：杜氏盐藻 14—3—3蛋白 原核表达 

分 类 号：Q781[生物学—分子生物学]