抗癌散乙酸乙酯部位指纹图谱的研究  

Research on the Fingerprint of Acetoacetate Extraction in Kangaisan

作　　者：楼一层[1] 阮班昭[1] 颜香凤[1] 杨翰[1] 黄芬[1] 

机构地区：[1]武汉理工大学化学工程学院,武汉430070

出　　处：《武汉理工大学学报》2008年第12期85-87,共3页Journal of Wuhan University of Technology

基　　金：国家自然科学基金(30440087305701843077276130870249)

摘　　要：采用高效液相色谱法建立抗癌散乙酸乙酯部位指纹图谱。利用硅胶层析进行纯化分离,以Kromasil 100A C18色谱柱,采用乙腈和磷酸水溶液为洗脱液进行梯度洗脱,流速为0.7 mL/min,柱温为35℃,检测波为323 nm。根据选择的色谱条件制订了不同批号抗癌散的指纹图谱。根据这8批抗癌散药材的分析结果可看出,指纹图谱相似度较高,该方法操作简便稳定,重复性好。说明不同批抗癌散药材的化学组成一致性较好,质量稳定。为抗癌散的进一步研究提供理论依据。High performance liquid chromatography（HPLC） was used to establish the fingerprint of acetoaeetate extraction of Kangaisan. Column chromatography properties was used to separate and purify the chemical constituents. The Kromasil 100A C18 chromatographic column with the conditions was chose as follows ： velocity of flow was 0.7 mL/min , the column temperature was 35 ℃ and the detective wave length was 323 nm. A good fingerprint of Kangaisan were described out. The result showed that eight groups Kangaisan medicinal materials had high recurrence , which indicated that the chemical elements in different origin of Kangaisan medicinal materials were similar. It was concluded that the quality of Kangaisan compound was steady.

关 键 词：抗癌散 指纹图谱 高效液相 

分 类 号：TQ463.23[化学工程—制药化工]