犬细小病毒四川株的分离与鉴定  

Isolation and identification of canine parvovirus Sichuan strains

作　　者：戈锐[1,2] 彭广能[1] 夏咸柱[2] 杨松涛[2] 苏建青[2,3] 邹啸环[2] 冯娜[2,3] 马晓平[1] 石锦江[1] 

机构地区：[1]四川农业大学动物科技学院,四川雅安625014 [2]军事医学科学院军事兽医研究所,吉林长春130062 [3]吉林大学畜牧兽医学院,吉林长春130062

出　　处：《中国兽医学报》2008年第12期1369-1373,共5页Chinese Journal of Veterinary Science

基　　金：全军医药卫生科技攻关课题“军犬重要疫病联合重组活疫苗的研发”课题(06G138)

摘　　要：采集5份患出血性肠炎犬的粪便,通过猫肾细胞(F81)传代培养后,成功分离到2株病毒。对2株病毒进行病毒形态学、理化学、血凝试验、PCR鉴定与分子生物学系统鉴定后,证实分离株是犬细小病毒,通过VP2结构蛋白测序分析表明,新分离到的2株细小病毒抗原型分别属于CPV-2a和CPV-2b。To better know the prevalence of CPV in China, five stool samples from clinical dogs with haemorrhagic enteritis in animal hospital were collected. Two strains of virus were isolated by passaging in F81 cells＇. After a series of systematic indentification,the virus were demonstrated to be canine parvovirus. According to the result of sequence analysis of VP2 gene,the antigenic types of the new isolated CPVs belong to CPV-2a and CPV-2b respectively.

关 键 词：犬细小病毒 分离 鉴定 

分 类 号：S857.65[农业科学—临床兽医学] S829.2[农业科学—兽医学]