维生素E缺乏对雏鸡脾脏淋巴细胞Caspase-3活性及Caspase-3 mRNA表达的影响  

Effects of VE deficiency on activity of Caspase-3 and expression of Caspase-3 mRNA of splenic lymphocytes in chicken

作　　者：张金龙[1] 李艳飞[1] 王奔[1] 封家旺[1] 

机构地区：[1]东北农业大学动物医学院,黑龙江哈尔滨150030

出　　处：《中国兽医学报》2008年第12期1438-1440,共3页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30471279);黑龙江省博士后科研启动金资助项目(LBH-Q05024)

摘　　要：用低维生素E(VE)和添加不饱和脂肪酸的日粮饲喂雏鸡建立VE缺乏雏鸡模型,采用比色法和半定量RT-PCR法分别检测脾脏淋巴细胞Caspase-3活性及Caspase-3 mRNA的表达水平。结果显示,VE缺乏组雏鸡脾脏淋巴细胞Caspase-3活性和Caspase-3 mRNA丰度高于对照组,组间差异均极显著(P<0.01)。结果表明,Caspase-3活性及Caspase-3 mRNA丰度的改变,是VE缺乏雏鸡脾脏淋巴细胞凋亡的调控机制之一。The activity of Caspase-3 and expression of Caspase-3 mRNA of splenic lymphocytes in the chicken with Vitamin E （VE） deficiency were detected by colorimetry and semi-quantitative reverse transcriptase-polymerase chain reaction （RT-PCR）. The results showed that the activity of Caspase-3 and abundance of Caspase-3 mRNA was higher in VE deficiency chicken than those of control （P〈0.01）. The results indicate that the changes of Caspase-3 activity and Caspase-3 mRNA abundance are one of the regulation mechanisms on lymphocyte apoptosis in the chicken with VE deficiency.

关 键 词：维生素E 脾脏 淋巴细胞 CASPASE-3 CASPASE-3 mRNA 

分 类 号：S816.7[农业科学—饲料科学] S852.2[农业科学—畜牧学]