黄河下游同域山羊种群mtDNA D-环多态性及系统发育  

MtDNA D-loop polymorphism and phylogeny of sympatric goat populations in lower valley of Yellow river

作　　者：侯磊[1] 王建民[1] 李培培[1] 李珏[1] 邢凤[1] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018

出　　处：《中国兽医学报》2008年第12期1474-1479,共6页Chinese Journal of Veterinary Science

基　　金：山东省肉羊良种工程项目(SDLZ05RY0203);科技部科技成果转化基金项目(05EFN213700167)

摘　　要：对黄河下游7个山羊种群共59个个体的mtDNAD-N533bp序列进行分析，发现59个多态位点，其中单一多态位点21个，简约信息位点38个，分别占全序列的11．7％、3．94％和7．13％。确定了37种单倍型，崂山奶山羊1个个体还发现了1处5碱基的缺失。各群体单倍型多样度为0．7857～0．9697，核苷酸多样度为1．27％～1．73％，遗传多样性较丰富。构建系统发育树将37种单倍型分为2个分支，由此推断黄河下游山羊资源至少具有2个母系起源，角猾羊与A类型聚为1支，7个群体具有角猾羊起源，未发现捻角山羊对7个群体有贡献的证据。错配分析表明，A类型经历过群体扩张，B类型未经历过群体扩张。并通过遗传距离分析了各群体的遗传分化。The partial mtDNA D-loop sequences of 59 individuals from 7 Shandong indigenous goat populations were analyzed. 59 polymorphic sites are defined,among which singleton polymorphic sites are 21, parsimony informative sites are 38,account for 11.7% ,3.94% and 7.13% of the whole sequence,respectively. In addition to a deletion of 5 bp fragment in one individual from LSN,37 haplotypes were defined. The haplotype diversity and nucleotide diversity are 0. 785 7-0. 969 7 and 1.27 %-1.73%, respectively, which shows abundant genetic diversity. Phylogenetic analysis of 37 haplotypes defined two major lineages, which suggests goats might have two maternal origins, C. aegagrus clustered with lineage A, these 7 populations might originate from C. aegagrus,whereas no evidence for C. falconeri contributed to the origin of the 7 populations was found. Mismatch analysis showed lineage A had experienced population expansion,whereas lineage B hadn＇t experienced population expansion. Genetic differentiation was also analysed by genetic distance.

关 键 词：山羊 MTDNA D-环 单倍型 系统发育 遗传分化 

分 类 号：S827[农业科学—畜牧学] S852.2[农业科学—畜牧兽医]