黄淮白山羊孤雌胚胎的体外培养  

Development of Huanghuai-white goat parthenogenetic embryos in vitro

作　　者：李斌[1,2] 韩伟[1] 方俊顺[1] 刘健明[1] 赵焕[1] 曹陈冲[1] 陶勇[1] 章美林[1] 章孝荣[1] 

机构地区：[1]安徽农业大学动物科技学院,安徽合肥233036 [2]海南省生殖医学中心,海南海口570102

出　　处：《中国兽医学报》2008年第12期1480-1482,共3页Chinese Journal of Veterinary Science

基　　金：安徽省“十五”重大科技专项(04003005);安徽省“十一五”科技攻关项目(06013051A)

摘　　要：采用离子霉素和6-DMAP对黄淮白山羊体外成熟卵母细胞进行联合激活,分别在不同的培养体系进行体外培养,观察孤雌胚胎的发育情况。培养体系分别为:无共培养条件下,M199(10%FCS)、CR1aa和HTF+P1;颗粒细胞共培养条件下,CR1aa、HTF+P1和SOFaa。结果发现,无共培养条件下,CR1aa和HTF+P1组孤雌胚胎的卵裂率显著高于M199组(P<0.05),但3组均未有囊胚;共培养条件下,HTF+P1组的卵裂率显著高于CR1aa和SOFaa组(P<0.05),而CR1aa组的囊胚率却显著高于其他2组(P<0.05)。综合试验结果说明,CR1aa联合颗粒细胞共培养能够获得较好的培养效果,适用于山羊孤雌胚胎的体外培养。In vitro matured H uanghuai-white goat oocytes were activated with ionomycin and 6-DMAP,then the parthenogenetic embryos were cultured in M199 （10% FCS）, CR1aa, HTF＋ P1, CR1aa with cumulus co-culture, HTF＋P1 with cumulus co-culture snd SOFaa with cumulus co-culture respectively. The result showed that without cumulus co-culture no embryos in all media developed to the blastulas,and the cleavage rate of embryos in M199 was significantly lower than that of the others （P〈0.05） ,with cumulus co-culture the cleavage rate embryos in HTF＋ P1 was significantly higher than that of the others（P〈0.05）, but the blastula rate in CR1aa was significantly higher than that of the others（P〈0.05）. It is concluded that CR1aa with cumulus co-culture system may be better suitable for goat parthenogenetic embryos culture in vitro.

关 键 词：山羊 孤雌激活 体外培养 

分 类 号：S827[农业科学—畜牧学] Q813.7[农业科学—畜牧兽医]