青藤碱对佐剂性关节炎大鼠炎症免疫功能的影响  

Effect of Sinomenine on Immune Function of Adjuvant Arthritis Rats

作　　者：陈方军[1] 叶丽[2] 胡伟[2] 张荣宜[3] 程远华[3] 

机构地区：[1]安庆医药高等专科学校临床系,安徽安庆246003 [2]安庆市立医院,安徽安庆246003 [3]安徽医科大学组织胚胎学教研室,安徽合肥230032

出　　处：《广州中医药大学学报》2008年第5期421-424,共4页Journal of Guangzhou University of Traditional Chinese Medicine

摘　　要：【目的】观察青藤碱(SINO)对佐剂性关节炎(AA)大鼠炎症免疫功能的影响。【方法】选用SD大鼠随机分为正常组,模型组,SINO低、中、高剂量组(剂量分别为60、120、240 mg/kg),雷公藤多苷(TPT)组(剂量为30 mg/kg);除正常组外,其他组均采用弗氏完全佐剂足跖皮内注射复制AA模型;各组于造模后第12天开始给药,连续12 d;观察不同时间段的大鼠足趾肿胀度及关节评分;采用放射免疫法检测滑膜细胞白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)的含量;观察各组大鼠滑膜细胞的超微结构;采用逆转录—聚合酶链反应检测各组滑膜细胞IL-1β mRNA、TNF-α mRNA的表达。【结果】各剂量组SINO均可不同程度降低大鼠足趾肿胀度和关节炎评分,抑制滑膜细胞产生IL-1β、TNF-α,降低滑膜细胞IL-1β mRNA、TNF-α mRNA的表达(P<0.05或P<0.01),恢复AA大鼠滑膜细胞的形态。【结论】SINO治疗类风湿性关节炎的作用可能与其能抑制滑膜细胞分泌炎性介质,恢复滑膜细胞形态有关。Objective To observe the effect of sinomenine （SINO） on immune function of adjuvant arthritis （AA） rats. Methods SD rats were randomized into normal group, model group, SINO groups （treated with gastric gavage of SINO at the doses of 60, 120 and 240 mg/kg respectively）, and glucosides of Tripterygium Wilfordii （ 30 mg/kg） group. Except the normal group, the rats in other groups received subcutaneous injection of the complete Freund＇s adjuvant 0. 1mL into the left hind foot to induce AA. The medication began from 12 days after the modeling and lasted 12 days. The pedal swelling and joint function scores were observed in different time. Radioimmunoassay was used to detect the contents of interleukin-1 β （IL-1 β） and tumor necrosis factor α（TNF-α） in synovial cells. Expression of IL-1β and TNF-α mRNA was examined by reverse transcription-polymerase chain reaction （RT-PCR） assay. Results SINO at different concentrations decreased the pedal swelling and arthritis scores to various degrees, inhibited the production of IL-1β and TNF-α in the synovial cells, reduced the expression of IL-1β and TNF-α mRNA, and recovered the normal histological features of synovial cells in AA rats. Conclusion The therapeutic mechanism of SINO for rheumatoid arthritis may be related with the inhibition of secretion of inflammatory mediators in synovial cells, and with the recovery of histological features of synovial cells.

关 键 词：青藤碱/药理学 关节炎 类风湿/中药疗法 关节炎 类风湿/免疫学 滑膜细胞/超微结构 疾病模型 动物 大鼠 

分 类 号：R285.5[医药卫生—中药学]