补肾生精法对老龄大鼠睾丸蛋白表达谱的影响  

Effect of Therapy of Tonifying Kidney and Replenishing Essence on Protein Expression Profile in Testes of Aged Rats

作　　者：陈通文[1] 曾金雄 王海鹰[1] 黄锦燕[1] 

机构地区：[1]广东省中医院珠海医院男科,广东珠海519015 [2]福州东方医院,福建福州350000

出　　处：《广州中医药大学学报》2008年第5期442-445,共4页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省自然科学基金项目(编号:06028730)

摘　　要：【目的】观察补肾生精法对老龄大鼠睾丸蛋白表达谱的影响。【方法】选用20只22月龄老龄雄性SD大鼠随机分为2组,每组10只,分别为空白对照组和补肾生精组;补肾生精组给药剂量为0.05 g.kg-.1d-1,连续灌胃给药60 d。取大鼠右侧睾丸,采用双向凝胶电泳法分析空白对照组和补肾生精组大鼠睾丸差异表达蛋白。【结果】与空白对照组比较,补肾生精组大鼠睾丸中有7种蛋白表达发生了显著变化,其中4种蛋白表达上调,3种下调。对7种差异表达蛋白点胶内酶切后进行质谱分析,其中5种蛋白分别鉴定为磷脂酰乙醇胺结合蛋白(phosphatidylethanolamine-binding protein,PEBP)、热休克蛋白8(heat shock 70 kDa protein 8,HSP7C)、磷酸丙糖异构酶(triose-phosphate isomerase,TPIS)、3-磷酸甘油醛脱氢酶(glyceraldehyde-3-phosphate dehydrogenase,GAPDH)、谷胱苷肽-S-基转移酶1(glutathione S-transferase Mu 1,GSTM1-1)。【结论】补肾生精法延缓衰老的作用可能与其能调节老龄大鼠睾丸蛋白表达有关。Objective To observe the effects of therapy of tonifying kidney and replenishing essence on protein expression profile in testes of aged rats. Methods Twenty male SD rats with 22 months old were equally randomized into the blank control group and the treatment group. The treatment group received gastric gavage of herbs at the dose of 0. 05g · kg^-1·d^-1, which has the actions of tonifying kidney and replenishing essence. The treatment lasted 60 days. After treatment, the right testes were taken out from the rats, and the differentially expressed proteins in the rats testes of two groups were analyzed by two-dimensional gel electrophoresis （2-DE）. Results Compared with the model group, obvious changes were found in 7 kinds of differentially expressed proteins in rats testes of the treatment group, of which 4 kinds were up-regulated and 3 down-regulated. After digestion and identification by the matrix-assisted laser desorption/ionization-time of flight （MALDI-TOF） mass spectrum, 5 kinds from the 7 kinds of proteins were identified as phosphatidyl ethanolamine-binding protein （PEBP） , heat shock 70 kDa protein 8, triose-phosphate isomerase, glyceraldehyde-3-phosphate dehydrogenase （GAPDH）, and glutathione S-transferase Mu 1 （GSTMI-1）. Conclusion The anti-aging mechanism of therapy of tonifying kidney and replenishing essence is probably related with the regulation of testis protein expression in aged rats.

关 键 词：补肾生精法/治疗应用 衰老/中药疗法 蛋白质组 疾病模型 动物 大鼠 

分 类 号：R285.5[医药卫生—中药学]