灵芝多糖对前列腺素E2抑制小鼠脾细胞增殖及IL-1α,IL-2 mRNA表达的拮抗作用  

Counteraction of Ganoderma Lucidum Polysaccharide on the Inhibition of Mice Splenocyte Proliferation and IL-1α and IL-2 mRNA Expression Induced by Prostaglandin E2

作　　者：张群[1] 雷林生[1] 余传林[1] 吴曙光[1] 

机构地区：[1]南方医科大学药物研究所,广东广州510515

出　　处：《广州中医药大学学报》2008年第6期549-552,556,共5页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省自然科学基金资助项目(编号:04020361)

摘　　要：【目的】观察灵芝多糖(GLB)对前列腺素E2(PGE2)抑制小鼠脾细胞增殖及IL-1α、IL-2 mRNA表达的拮抗作用。【方法】采用混合淋巴细胞培养反应作为实验模型,四甲基偶氮唑盐(MTT)法检测细胞增殖程度,半定量逆转录聚合酶链反应(RT-PCR)法检测IL-1α及IL-2 mRNA的表达水平。【结果】PGE2连续作用48 h后,脾细胞增殖反应受到不同程度的抑制作用,当PGE2浓度在10μmol/L以上时差异有显著性意义(P<0.01);固定PGE2的浓度为20μmol/L,并合用不同浓度的GLB(25、50、100、200 mg/L),当GLB为100 mg/L以上浓度时可部分对抗PGE2(20μmol/L)的抑制作用(P<0.05);培养8 h及12 h后,PGE2(20μmol/L)可抑制IL-1α及IL-2 mRNA的表达,GLB为100 mg/L以上浓度时可部分拮抗PGE2的抑制作用(P<0.05或P<0.01)。【结论】灵芝多糖可部分拮抗前列腺素E2对小鼠脾细胞增殖及IL-1α和IL-2 mRNA表达的抑制作用。Objective To observe the counteraction of Ganoderma lucidum polysaccharide （GLP） on the inhibition of mice splenocyte proliferation and IL-1α and IL-2 mRNA expression induced by prostaglandin E2 （PGE2）. Methods Mixed lymphocyte culture reaction （MLR） method was used for the experiment. The lymphocyte proliferation was determined by MTT method, and the levels of IL-1 α and IL-2 mRNA expression were evaluated by semi-quantitative reverse transcriptase polymerase chain reaction （RT-PCR）. Results After co- culture with PGE2 for 48 hours, the splenocyte proliferation was inhibited to some extent, and the difference was significant when the concentration of PGE2 was over 101μmol/L （ P 〈 0. 01 compared with that before treatment with PGE2）. The results co-culture with PGE2 at 20p^mol/L and GLP at different concentrations of 50, 100 and 200 mg/L showed that GLP at 100 mg/L or above had partial counteraction on PGE2-induced inhibition of splenocyte proliferation. After co-culture with PGE2 at 20μmol/L and GLP at 100 mg/L or above for 8 and 12 hours, the IL-lα mRNA expression and IL-2 mRNA expression were inhibited in mice （P 〈0. 05 or P 〈0.01 compared with that before treatment with GLP ）. Conclusion GLP can counteract the inhibition of mice splenocyte proliferation and IL-lα and IL-2 mRNA expression induced by PGE2.

关 键 词：灵芝多糖/药理学 肿瘤/中药疗法 肿瘤/免疫学 细胞培养 

分 类 号：R285.5[医药卫生—中药学]