五味地龙汤对哮喘豚鼠BALF中EOS和肺支气管组织形态学的影响  

Effect of Wuweidilong Decoction on BALF EOS and Histomorphology of Arteriae Pulmonalis of Guinea Pigs with Asthma

作　　者：张德新[1] 李芳梅[2] 李祥华[1] 潘丰满[1] 黄江荣[1] 

机构地区：[1]长江大学医学院,湖北荆州434100 [2]湖北省荆州市中心医院,湖北荆州434100

出　　处：《四川中医》2008年第12期17-21,共5页Journal of Sichuan of Traditional Chinese Medicine

基　　金：湖北省教育厅科学技术研究项目(编号:B200512006)

摘　　要：目的:观察五味地龙汤对实验性支气管哮喘豚鼠支气管肺泡灌洗液(BALF)中嗜酸性粒细胞(EOS)的计数和肺、支气管组织形态学的影响。方法:用OVA腹腔注射及气道吸入激发建立哮喘豚鼠模型,对支气管肺泡灌洗液离心后计数BALF中EOS。光镜观察其肺支气管病理组织学变化。结果:①一般情况:哮喘模型组豚鼠均出现不同程度的呼吸次数增加、张口等呼吸困难症状,各药物组豚鼠OVA激发后,地塞米松组和五味地龙汤大剂量组没有出现明显不适症状。②BALF中EOS计数显示:各组与哮喘模型组EOS(15.57±1.51)个/mm3比较,差异均有非常显著的统计学意义(P<0.01)。五味地龙汤大剂量组和地塞米松组与正常对照组EOS(0.83±0.75)个/mm3比较,差异均无统计学意义(P>0.05)。哮喘性模型组BALF中EOS数明显增加。③光镜观察:模型组见支气管黏膜下层充血水肿,嗜酸性粒细胞灶状浸润,腔内可见部分粘液及渗出物。地塞米松组:气管及支气管管壁结构正常,无炎性渗出。五味地龙汤大剂量组:气管管壁结构逐渐恢复,炎症减轻,水肿明显消失。管腔炎性渗出物明显减少。结论:五味地龙汤可能通过减少气道EOS浸润,减轻炎性渗出,抑制杯状细胞和平滑肌增生,抑制气道重塑,从而发挥平喘的作用。Objective： To study the effect of wuweidilong decoction on the number of eosinophil （EOS） in bronchoalveolar lavage fluid （BALF） and histomorphologieal change of lungs and bronchi in guinea pigs with asthma. Methods： The model of asthma was induced by intra-peritoneal injection and inhalation of OVA. EOS was counted after BALF eentrifugation. The histomorphological changes of lungs and bronchi were observed through light microscope. Results： （1） General symptoms： In model group, there appeared some dyspnea symptoms such as increased perspiration frequency and breathing with mouth opening. However, there were no dyspnea symptoms in treatment group after treated with dexamethasone or Wuweidilong decoction. （2） EOS counts： There was significantly statistical difference （P 〈 0.01） in EOS counts between treatment groups and model group 115.57±1.51 /mm^2） . Them was no significant difference in EOS counts between high dose group of Wuweidilong decoction and dexamethasone group and normal control group （0. 83±0.75 / mm^2） （P 〉 0.05） . EOS count of model group increased significantly. （3） Observation through light microseope： In model group, there presented congestion and edema in bronchial sub-mucosa, focal infiltration of eosinophil cells, and mucus and exudatum in intracavity. In dcxamethasone group, there was no change in the wall of trachea and bronchi. In high dose group of Wuweidilong： Structure of tracheal wall recovered gradually. Inflammation was alleviated and edema disappeared. Inflammatory exudates in trachea were decreased obviously. Conclusion： Wuweidilong decoction has a function of antiathma through alleviating inflammatory exudates, restraining the proliferation of goblet ceils and smooth muscle, and restraining airway remodeling .

关 键 词：五味地龙汤 哮喘 支气管肺泡灌洗液 嗜酸性粒细胞 病理 

分 类 号：R285.5[医药卫生—中药学]