大黄药材及饮片的土大黄苷新检查方法  

Study on the Rhaponticin Examination of Rhubarb and Processed Rhubarb

作　　者：孙佩[1] 李敏[2] 张光仁 唐艳萍[2] 吴发明[2] 

机构地区：[1]四川省农业科学院经济作物育种栽培研究所,四川简阳641400 [2]成都中医药大学,四川成都610075 [3]达州食品药品检验所,四川达州635000

出　　处：《四川中医》2008年第12期59-60,共2页Journal of Sichuan of Traditional Chinese Medicine

基　　金：科技部863项目"大黄配方颗粒质量标准规范化示范研究"(编号为2001BA701A40-5)

摘　　要：目的:建立可靠的土大黄苷检查方法。方法:对传统方法、中国药典方法检查土大黄苷进行了研究,在此基础上建立了以土大黄苷为对照的薄层色谱法和高效液相色谱法检查大黄药材及饮片中土大黄苷。结果:土大黄苷薄层检查法对大黄药材及饮片的检查均适用。采用高效液相色谱法对番泻苷和土大黄苷在同一色谱条件下测定,不仅能鉴别正伪品大黄药材及饮片,还能检出掺伪土大黄的大黄药材及饮片。结论:该薄层色谱法和高效液相色谱法准确可靠,可用于大黄药材及饮片的质量控制。Objective： To establish a reliable method for Rhaponticin examination. Methods： On the basis of traditional method and method set in Chinese Pharmacopoeia （2005）, TLC method and HPLC method for Rhaponticin examination were established. Results： TLC method could be used for Rhaponticin examination in both Rhubarb and Processed Rhubarb. HPLC method could test Sennoside and Rhaponticin in the same chromatographic conditions, and could not only identify the true or falas Rhubarb and Processed Rhubarb, but also examine the purity. Conclusion： The TLC method and HPLC method are accurate and reliable. It＇s necessary for the quality control of Rhubarb and Processed Rhubarb.

关 键 词：大黄 土大黄苷 薄层色谱法 高效液相色谱法 

分 类 号：R285[医药卫生—中药学]