麦谷蛋白Glu-1和Glu-3位点基因等位变异对籽粒聚合体蛋白粒度分布的影响  

The Effects of Allelic Variation at Glutein Subunit Loci Glu 1 and Glu 3 on the Size Distribution of Polymeric Protein

作　　者：赵会贤[1] 胡胜武[1] 吉万全[1] DarylMares 

机构地区：[1]陕西省农业科学院 [2]澳大利亚悉尼大学植物育种研究所

出　　处：《中国农业科学》1998年第1期69-75,共7页Scientia Agricultura Sinica

摘　　要：用单向一步ＳＤＳ－ＰＡＧＥ方法分析表明小麦品种Ｓｕｎｅｃａ和Ｃｏｏｋ在麦谷蛋白５个亚基位点（Ｇｌｕ－Ｂ１，Ｇｌｕ－Ｄ１，Ｇｌｕ－Ａ３，Ｇｌｕ－Ｂ３和Ｇｌｕ－Ｄ３）均含不同等位基因。选用Ｓｕｎｅｃａ×Ｃｏｏｋ的Ｆ４代群体中在麦谷蛋白亚基位点均为纯合基因的６０个系，研究麦谷蛋白亚基位点基因等位变异对小麦籽粒聚合体蛋白粒度大小分布（用ＳＥ－ＨＰＬＣ测定）的影响。结果表明：Ｇｌｕ－Ｂ１位点等位基因ｉ和ｕ，Ｇｌｕ－Ｄ１位点等位基因ｄ和ａ，对籽粒聚合体蛋白粒度大小相对分布（用不溶聚合体蛋白占总聚合体蛋白含量的百分数表示，即ＵＰＰ％）的效应存在显著差异，含Ｇｌｕ－Ｂｌｕ基因和Ｇｌｕ－Ｄｌｄ基因的系分别比含Ｇｌｕ－Ｂｌｉ和Ｇｌｕ－Ｄｌａ的系有较大的ＵＰＰ％；同样，Ｇｌｕ－Ａ３位点等位基因ｄ和ｂ，Ｇｌｕ－Ｂ３位点等位基因ｈ和ｂ，对籽粒聚合体蛋白粒度大小相对分布的效应显著不同，Ｇｌｕ－Ａ３ｂ基因和Ｇｌｕ－Ｂ３ｂ基因与较大的ＵＰＰ％有关。Ｇｌｕ－Ｄ３位点等位基因ｅ和ｂ对ＵＰＰ％无显著影响。Ｇｌｕ－１位点和Ｇｌｕ－３位点间对籽粒聚合体蛋白大小相对分布的影响存在累加和互作效应。Separation of glutenin subunits by one step 1 D SDS PAGE revealed that the parents Suneca and Cook had contrasting alleles at each of the five glutenin subunits loci (Glu B1,Glu D1,Glu A3,Glu B3 and Glu D3). Thus, a set of 60 lines homozygous of these loci from the F 4 progeny population was chosen to study the effects of allelic variation at glutenin subunit on the size distributions of polymeric protein(measured by SE HPLC) from wheat seed.The results showed that HMW glutenin subunit alleles (i and u)at the Glu B1 locus and alleles (d and a)at the Glu D1 locus significantly affect the relative size distributions of polymeric protein (i.e.percentage of unextractable polymeric protein in the total polymeric protein,or UPP%). Alleles Glu Blu and Glu Dld were associated with greater UPP% value. Similarly,the alleles (d and b) at the Glu A3 locus and alleles (b and h) at the Glu B3 locus gave significantly different relative size distributions of polymeric protein,the progeny with allele Glu A3b and Glu B3b had greater portion of unextractable polymeric protein than the progeny with allele Glu A3d and Glu B3h,respectively.However,alleles(e and b) at the Glu D3 locus did not give significantly different UPP% values.The effect of alleles at Glu 1 and Glu 3 locus on the relative size distributions of polymeric protein appears cumulative and interactive.

关 键 词：基因等位变异 聚合体蛋白粒度 小麦 育种 

分 类 号：S512.103.2[农业科学—作物学]