大蒜(Allium sativum L.)球状体形态发生中的细胞组织学研究(英文)  

INDUCTION AND UTILIZATION OF GLOBULAR BODIES ON CALLI FROM OF GARLIC(Allium sativum L.)LEAF EXPLANTS--STUDY OF CELLULAR HISTOLOGY IN MORPHOGENESIS

作　　者：郑海柔[1] 沈敏健[2] 钟维瑾[3] 张智奇[3] 周音[3] 

机构地区：[1]上海市农业科学院园艺研究所,上海201106 [2]上海师范大学生物系,上海200234 [3]上海市农业科学院作物育种栽培研究所,上海201106

出　　处：《上海农业学报》1998年第1期33-38,共6页Acta Agriculturae Shanghai

基　　金：FAO/IAEA部分资助

摘　　要：大蒜叶片外植体在含有500mg／L水解酪蛋白、1000mg／L酵母提取物和2mg／L2，4－D的MS培养基上，产生可分离的球状体。球状体在含有2mg／LKT和0．5mg／LIAA的MS培养基上幼苗诱导率约91．7％。培养基中5％的蔗糖浓度可促进球状体的产生。本文对球状体作了形态解剖描述。球状体可在大蒜快速繁殖技术上加以应用。Detachable globular bodies were produced on calli induced from leaf garlic explants on MS medium containing 500 mg/L casein hydrolysate+1000 mg/L yeast extract+2 mg/L 2,4-D. Plantlets were regenerated from globular bodies cultured on MS medium containing 2 mg/L KT+0. 5 mg/L IAA. The frequency of plantlets was about 91. 7%. Globularbody production was accelerated on the medium supplemented with 5% sugar. Morphologicaland anatomical description of gloLular bodies was also made in this paper. Globular bodiescould be used in a rapid micropropagation technique for garlic.

关 键 词：大蒜 组织培养 球状体诱导 快速繁殖 细胞组织学 

分 类 号：S633.403.5[农业科学—蔬菜学]