黄荆子乙酸乙酯提取物体内外对胃癌SGC-7901细胞作用的研究  

Effects of the extract from semen viticis negundo with acetoactate on human gastric carcinoma SGC-7901 cell in vitro and in vivo

作　　者：韩家凯[1] 焦东晓[2] 曹建国[3] 封萍[1] 刘新福[1] 庄英帜[1] 

机构地区：[1]南华大学附属第一医院肿瘤内科,湖南衡阳421001 [2]南华大学附属第一医院肿瘤外科,湖南衡阳421001 [3]南华大学肿瘤研究所,湖南衡阳421001

出　　处：《中国药理学通报》2008年第12期1652-1656,共5页Chinese Pharmacological Bulletin

摘　　要：目的研究黄荆子乙酸乙酯提取物(EVn-50)在体内外对人胃癌SGC-7901细胞的作用。方法细胞计数法检测EVn-50对人胃癌SGC-7901细胞生长增殖的抑制作用,并绘制细胞生长曲线;平板克隆试验测定细胞集落形成率;建立SGC-7901裸鼠异种移植瘤模型,绘制移植瘤生长曲线,计算经EVn-50治疗后的肿瘤抑制率;光镜和电镜观察肿瘤组织病理变化。结果在体外,1、10、100mg·L-1的EVn-50均能抑制人胃癌SGC-7901细胞生长和增殖,呈浓度和时间依赖性;与对照组比较,细胞集落形成率明显下降(P<0.05);在体内,EVn-50能够抑制SGC-7901细胞裸鼠异种移植瘤生长,5、10、20mg·kg-1的EVn-50对移植瘤的瘤重抑制率分别为32%、43%和56%,且呈浓度依赖性;病理学观察结果,EVn-50可引起SGC-7901细胞坏死,诱导SGC-7901细胞凋亡。结论EVn-50在体内外均可抑制SGC-7901细胞生长和增殖,并在体内诱导其凋亡。Aim To study the effects of EVn-50 on human gastric carcinoma SGC-7901 cells in vitro and in vivo. Methods Human gastric carcinoma SGC-7901 cells were cultured in vitro. The inhibitory rate of cells was determined by cell counting and the cell growth curve was made. Plate clone formation assay was carried out to detect the phenotypes of colony formation. Trail of human gastric carcinoma xenografts in nude mouse model was used to draw the transplant tumor growth curve and test inhibition rate of EVn-50 on human gastric carcinoma. The histopathological changes were observed by lightmicroscopy and electronmicroscopy. Results In vitro, EVn-50 at 1,10,100 mg· L^-1 inhibited the growth and proliferation of SGC-7901 cells in a dose-dependent manner and a time-dependent manner; The colony-forming rate was reduced drastically compared with control group （ P 〈 0.05 ）. In vivo,EVn-50 inhibited the xenograft growth of SGC-7901 in nude mice, in a dose-dependent manner. The tumor weight inhibitory rate of EVn-50 at 5,10,20 mg·kg^-1 was 32% ,43%,56% respectively, in a dose-dependent manner. The pathologieal alterations were observed. In EVn-50 groups, degrees of necrosis in tumor tissues were markedly inereased respeetively eompared with the model group and celt apoptosis could be observed. Conclusion EVn-50 can significantly inhibit the growth and proliferation of gastric eareinoma cells SGC-7901 in vitro and in vivo, and induce apoptosis of SGC-7901 cells in vivo.

关 键 词：胃癌 黄荆子 黄荆子乙酸乙酯提取物 移植瘤 

分 类 号：R284.1[医药卫生—中药学] R329.24[医药卫生—中医学]