新生大鼠海马神经干细胞表达趋化因子受体CCR5的体外研究  

In vitro study of the expression of chemokine receptor CCR5 in neural stem cells of neonatal rat hippocampus

作　　者：丁鹏[1] 薛黎萍[2] 杨智勇[1] 王进昆[1] 余化霖[1] 冯曜宇[1] 冯忠堂[3] 

机构地区：[1]昆明医学院第一附属医院神经外科,云南昆明650032 [2]云南省第二人民医院眼科,云南昆明650021 [3]昆明医学院神经科学研究所,云南昆明650031

出　　处：《中华神经外科疾病研究杂志》2008年第6期485-488,共4页Chinese Journal of Neurosurgical Disease Research

摘　　要：目的探讨体外培养神经干细胞是否表达趋化因子受体CCR5。方法采用无血清方法分离、培养新生大鼠海马神经干细胞,细胞免疫荧光方法检测神经干细胞标志巢蛋白(nestin)表达及干细胞多向分化为神经元及胶质细胞的能力;后通过细胞免疫荧光及逆反转录酶-聚合酶链反应(RT-PCR)方法检测神经干细胞表达趋化因子受体CCR5的情况。结果体外培养新生大鼠海马神经干细胞呈nestin阳性,可分化为神经丝蛋白200(NF200)阳性神经元、胶质纤维酸性蛋白(GFAP)阳性星形胶质细胞及2,3-环核苷酸磷酸二酯酶(CNP)阳性少突胶质细胞,CCR5细胞免疫荧光及RT-PCR证实神经干细胞表达趋化因子受体CCR5。结论新生大鼠海马神经干细胞表达趋化因子受体CCR5,为进一步研究其体内、外迁移提供理论依据。Objective To explore whether neural stem ceils, in vitro, expressed chemokine receptor CCRS. Methods The neural stem cells were isolated from the hippocampus of neonatal rats and cultured in serum-free medium. Using immunoflurescence, the neural stem cells and its differentiation into neural and glial cell were identified. Then immunofluorescence and reverse transcription-polymerase chain reaction （RT-PCR） were employed to detect whether neural stem cells, in vitro, expressed chemokine receptor CCRS. Results The floating neural spheres, isolated from neonatal hippocampus, were nestin positive and could differentiate into cells to express characteristic antigen of neurons, astrocytes and oligodendrocytes. Iinmunofluorescence and RT-PCR showed that the neural stem cells expressed chernokine receptor CCRS. Conclusion Neural stem cells, in vitro, express chemokine receptor CCRS, which provides the theoretical evidence for further study of its migration.

关 键 词：神经干细胞 趋化因子 体外 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]