西安市结核病的病原学调查  

Bacteriological investigation of tuberculosis in Xi'an city

作　　者：张选民[1] 张媛媛[2] 曾令城[1] 王艳[2] 王西临[1] 赵秀芹[2] 杜新玲[1] 刘志广[2] 韩跃玲[1] 万康林[2] 

机构地区：[1]陕西省西安市疾病预防控制中心西安,710054 [2]传染病预防控制国家重点实验室中国疾病预防控制中心传染病预防控制所,北京102206

出　　处：《中国人兽共患病学报》2008年第12期1118-1121,共4页Chinese Journal of Zoonoses

基　　金：国家“十五”重点攻关项目2001DEA10007“中国重要医学生物资源的保藏与共享--我国重要细菌菌种的收集、保藏与共享”;西安市社会攻关项目“提高结核病发现率新技术的研究”(项目批准号GG06165)联合资助

摘　　要：目的调查西安市结核病的分枝杆菌菌种类型及其分布。方法采用抽样调查方法收集结核病患者的痰标本及其背景资料,用痰涂片、痰培养以及PCR方法检测,用鉴别培养基对分离菌株进行菌种鉴定。结果共收集574例拟诊结核病病例,实验室检测1602份痰标本。采用痰涂片、痰培养和PCR3种方法联合应用,阳性者260例,分枝杆菌检测阳性率45.30。137例分枝杆菌培养阳性者中,结核分枝杆菌复合体占73.72,其中结核分枝杆菌和牛分枝杆菌分别为67.88和5.84;11.68为非结核分枝杆菌,14.60为混合感染,其中11.68为结核分枝杆菌与牛分枝杆菌,2.92为结核分枝杆菌与非结核分枝杆菌混合感染。结论西安市结核病的致病菌复杂,包括结核分枝杆菌、牛分枝杆菌和非结核分枝杆菌,尤其是非结核分枝杆菌混合感染应予以高度重视。To investigate bacteriological prevalence situation and distribution of tubeculosis （TB） and to provide the basic data for TB control and prevention. The sputum samples and the background data from suspect patients with TB were collected by means of sampling epidemiological investigation in Xi＇an City. The bacteria in sputum samples were detected with smear slide microcopy, culture and polymerase chain reaction （PCR）, and mycobateriom strains isolated from the sputum samples were identified by the identification medium. 1602 sputum samples from 574 suspect patients with TB were detected. The positive rate of mycobacterium infected was 45.30 % with co-employment of the above three methods. In 137 cases with positive culture result, 67.88% were infected by Mycobacteriurn tuberculosis （M. tuberculosis）, 5. 84% by Mycobacterium bowls （M. boris） ,and 11.68% by non-tuberculosis mycobacterium （NTM）. Meanwhile, 14. 60% were mix-infected by M. tuberculosis, NTM and M. boris, in which 11. 68% were infected by M. tuberculosis and M. bovis and 2. 920/00 by M. tuberculosis and NTM. It is apparent that the etiological pathogens of TB in Xi＇an City is rather complicated, including M. tuberculosis, M. bovis and NTM, The infection with NTM and the Mixed infetion should not be ignored.

关 键 词：结核病 结核分枝杆菌 非结核分枝杆菌 病原学调查 

分 类 号：R378[医药卫生—病原生物学]