糖尿病大鼠回肠肌间神经丛mGluR1及mGluR5的表达  

Expression of metabotropic glutamate receptors mGluR1 and mGluR5 in the myenteric plexus of the diabetic rat ileum

作　　者：白艳华[1] 张军[1] 史红阳[1] 戴菲[1] 李芳伟[1] 

机构地区：[1]西安交通大学医学院第二附属医院消化科,陕西省西安市710004

出　　处：《世界华人消化杂志》2008年第33期3734-3739,共6页World Chinese Journal of Digestology

摘　　要：目的:了解糖尿病大鼠胃肠动力障碍时,回肠肌间神经丛有无形态学异常,以及第Ⅰ组代谢型谷氨酸受体mGluR1、mGluR5的表达变化,探讨谷氨酸能神经在糖尿病胃肠病变发生中的作用.方法:40只大鼠随机分为糖尿病组和对照组,给与高脂饮食结合腹腔注射STZ 30 mg/kg造糖尿病模型.运用肌间神经丛铺片观察谷氨酸能神经的分布及形态特征,并对其神经节和神经元进行定量研究,以及运用免疫荧光染色和RT-PCR方法观察大鼠回肠肠神经系统肌间神经丛的代谢型谷氨酸受体mGluR1、mGluR5的表达变化.结果:糖尿病大鼠肠神经系统肌间神经丛的谷氨酸能神经节和神经元的数目较对照组明显减少(mGluR1:4.5±3.1 vs 7.3±2.4;142.25±28.24vs 175.34±34.83,均P<0.05;mGluR5:4.3±2.1 vs 7.9±2.8,133.37±35.73 vs 168.34±32.66,均P<0.05),荧光强度较对照组减弱(mGluR1:145.23±28.78 vs 167.72±30.56,均P<0.05;mGluR5:141.54±18.46 vs 172.53±29.74,均P<0.05),mGluR1、mGluR5受体mRNA表达减少(1.05±0.27 vs 1.43±0.47,0.95±0.30vs 1.60±0.39,均P<0.01).结论:糖尿病大鼠回肠肠壁的肌间神经丛的神经节和神经元数目减少,以及兴奋性递质受体mGluR1、mGluR5的表达减少是导致胃肠道肌层兴奋性降低,肌层收缩减弱,引起糖尿病胃肠病变的一个重要机制.AIM：To find the morphologic abnormalities,if any,of myenteric plexus in diabetic rats,to explore the expressive alteration of the Group ⅠmGluRs（mGluR1,mGluR5）and to explore the role of glutamic acid energic nerve in enteric nervous system of diabetic gastroenteropathy.METHODS：Forty rats were randomly divided into diabetic group and control group.Gastric emptying and small intestine transit rate were measured,and the number of mGluR1 and mGluR5 receptors in diabetic rats was studied using fluorescence immunohistochemistry and RT-PCR.RESULTS：Eighteen weeks after the establish-ment of the diabetic rats model,gastric emptyingand gastrointestinal transit rate were delayed compared with control group.The number of ganglia and neurons was significantly decreased in diabetic rats compared with control group（mGluR1：4.5±3.1 vs 7.3±2.4,142.25±28.24 vs 175.34±34.83,both P〈0.05;mGluR5：4.3±2.1 vs 7.9±2.8,133.37±35.73 vs 168.34±32.66,both P〈0.05）.The fluorescence intensity of the recep-tors of mGluR1 and mGluR5 in the diabetic rats was weakened compared with control group（mGluR1：145.23±28.78 vs 167.72±30.56,both P 〈0.05;mGluR5：141.54±18.46 vs 172.53±29.74,both P〈0.05）,and mGluR1 and mGluR5 mRNA expressions were decreased in the diabetic rats（1.05±0.27 vs 1.43±0.47,0.95±0.30 vs 1.60±0.39,both P〈0.01）.CONCLUSION：Decreased glutamatergic gan-glia and neurons and reduced receptor expres-sion of mGluR1 and mGluR5 in myenteric plex-us might be one of the mechanisms of diabetic gastroenteropathy in rats.

关 键 词：肠神经系统 谷氨酸 代谢型谷氨酸受体mGluR1 代谢型谷氨酸受体mGluR5 

分 类 号：R587.2[医药卫生—内分泌] R574[医药卫生—内科学]