4个绵羊品种Leptin基因部分片段的SNPs多态性与生长发育性状的相关性分析  

Analysis on Associations of SNPs of Leptin Gene with Growth Traits in Four Sheep Breeds

作　　者：黄丹丽[1] 陈仁金[1] 杨章平[1] 毛永江[1] 李云龙[1] 田黛君 陈亮 赵晓勇 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009 [2]宁夏畜牧工作站,银川750000 [3]中宁县畜牧推广中心,中宁751200

出　　处：《畜牧兽医学报》2008年第12期1640-1646,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：江苏省自然科学基金(BK2003040);江苏省农业高技术项目(BG2007324)

摘　　要：利用PCR-SSCP技术对萨福克、陶赛特、得克塞尔及滩羊4个绵羊品种358个个体Leptin基因等2、3外显子进行多态性分析,共检测到7个SNPs,其中新发现5个SNPs。测序结果表明,在外显子2上无突变。内含子2上存在A99G、G115A、C150T、C171T位点。外显子3上,存在G271A;C316A;G387T位点。外显子3上的SNPs使编码的氨基酸发生变化。统计分析表明A99G、C150T和A99G+C150T位点与生长发育性状存在相关性。在A99G位点,Aa基因型初生质量、日增质量、体高、胸围和尻宽指标上均高于AA基因型,初生质量、日增质量和体高指标差异显著(P<0.05),胸围和尻宽指标差异极显著(P<0.01)。C150T和A99G+C150T位点结果一致,突变基因型日增重、体高、体长、胸围和尻宽指标均高于野生基因型,差异显著(P<0.05)。PCR-SSCP was applied to detect single nucleotide polymorphism （SNP） of exon 2 and exon 3 of the Leptin gene for 358 sheep, including Poll Dorsets （PD）, Suffolks （SF）, Texels （TX） and Tan sheep （Tan）. The polymorphisms were demonstrated, and exon 2 and exon 3 of leptin gene were sequenced. Seven SNPs were detected, five of which were new SNPs. There is no SNP in exon2, but four SNPs were detected in parts of intron 2. They are A99G, G115A, C150T and C171T. Three SNPs occurred in exon3, all of which resulted in amino acid changes. The association between some SNPs and growth traits were analyzed. The results showed that the average values of birth weight, daily gain and withers height of Aa were significantly higher than those of AA at A99G SNP（P〈0.05）, while the values of heart girth and rump width of Aa were very significantly higher than those of AA （P〈0.01）. The average values of daily gain, withers height, body length, heart girth and rump width of mutantions types were significantly higher than those of wide-types at C150T SNP （P〈0.05）. The results of A99G＋C150T polyorphic site were the same to C150T SNP.

关 键 词：绵羊 LEPTIN基因 SNPS 生长发育性状 

分 类 号：S826.2[农业科学—畜牧学]