山羊IGFBP-3基因的遗传分析及其与经济性状的关系  

Genetic Analysis of IGFBP-3 Gene and Its Association with Economic Traits in Goats

作　　者：李美玉[1] 李兰[1] 闵令江[1] 王建民[2] 

机构地区：[1]青岛农业大学动物科技学院,青岛266109 [2]山东农业大学动物科技学院,泰安271018

出　　处：《畜牧兽医学报》2008年第12期1647-1653,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：公益性行业(农业)科研专项(nyhyzx-07-037);青岛农业大学高层次人才启动基金(630515);青岛农业大学科研基金(610689)

摘　　要：用PCR-RFLP技术对7个山羊群体的501个个体进行了检测,结果发现:每个群体均存在XspⅠ多态,表现为3种基因型,均以杂合型AG居多,以GG最少,等位基因A和G在每个群体中基本稳定,7个群体均处于Hardy-weinberg平衡状态(P>0.05),且基因型在群体间的分布也不存在显著差异(P>0.05)。序列分析表明:扩增片段包括山羊IGFBP-3基因的部分exon2、完整intron2和exon3及部分intron3,与奶山羊、绵羊、奶牛相应序列的同源性依次为99.1%、98.1%、94.1%。同时与经济性状的最小二乘分析表明:波尔山羊及其杂交后代的该位点与断奶体质量和眼肌面积有关(P<0.05),对3月龄体长和胸围有极显著影响(P<0.01),与10月龄体长、胸围和12月龄体长相关(P<0.05),AG基因型均显著或极显著地大于GG型,且呈现AG>AA>GG的趋势。501 goat individuals in seven populations were detected by PCR-RFLP. The results showed the polymorphisms of Xsp I existed in every population and the number of the individuals with genotype AG was more than that with genotype AA while the number of genotype AA was more than that of GG in all the populations. At the same time, the results of X2 test indicated that all the populations were at Hardy-weinberg equilibrium （P〉0.05） and genotype distributions were not relative to populations （P〉0.05）. We also found that frequency of allele A was higher than that of allele G and the frequencies of allele A and G were nearly invariable in all populations. PCR products were sequenced and the result showed that the sequence contains partial exon2, complete intron2, exon3 and partial intron3. The homology of the gene sequences with and dairy goat, sheep, dairy cattle, were 99. 1%, 98. 1% and 94.1%, respectively. The correlations between genotypes at the gene locus and weaning weight, rib eye area, 10th month body length, 10th heart girth and 12th month body length were significant （P〈0. 05） and the associations were significant for 3rd month body length and 3rd heart girth （P〈0.01）. The genetic effects of genotype AG were significantly higher than those of genotype GG （P〈 0.05 for weaning weight and rib eye area while P〈0.01 for above five physical measure traits）. Further more, the effects of genotypes for above traits were AG〉AA〉GG.

关 键 词：山羊 IGFBP-3基因 PCR—RFLP 遗传分析 经济性状 

分 类 号：S823[农业科学—畜牧学] S827.2[农业科学—畜牧兽医]