能量水平和来源对后备母猪卵母细胞质量及相关基因表达的影响  

Effects of Dietary Energy Level and Source on Oocyte Quality and Gene Expression in Gilts

作　　者：王延忠[1] 吴德[1] 徐盛玉[1] 周东胜[1] 周平[1] 谭现义[1] 

机构地区：[1]四川农业大学动物营养研究所教育部动物抗病营养重点实验室,雅安625014

出　　处：《畜牧兽医学报》2008年第12期1671-1678,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：教育部创新团队计划(IRT0555);国家自然科学基金(30471257)

摘　　要：选择初始体质量(59±4.2)kg的长白×大白杂交母猪54头,采用3×2试验设计,研究日粮能量水平和来源对后备母猪卵泡发育和卵母细胞体外成熟以及排卵前卵丘-卵母细胞复合物(COCs)中生长分化因子-9(GDF-9)、骨形态发生蛋白-15(BMP-15)、促黄体素受体(LHR)、促卵泡素受体(FSHR)mRNA表达的影响。3个能量水平分别为NRC推荐能量需要的87.5%、100%和112.5%;每个能量水平分别以淀粉和油脂为主要能量来源。试验母猪在第2个发情期的第19天屠宰。结果表明:随能量水平升高,大卵泡数增多(P<0.05),能量来源对卵泡数量没有显著影响(P>0.05);高能量水平下卵丘扩散程度(P<0.05)和卵母细胞成熟的比例最高(P<0.01),能量来源对卵丘扩散程度影响差异不显著(P>0.05),淀粉组卵母细胞成熟的比例高于油脂组(P<0.05);基因分析表明,饲喂低能量水平组的GDF-9和BMP-15 mRNA的表达量最高(P<0.05),淀粉组上调GDF-9 mRNA的表达(P<0.05),能量来源对BMP-15 mRNA表达影响差异不显著;各处理小卵泡的卵丘-卵母细胞复合物中GDF-9和BMP-15 mRNA表达量高于大卵泡(P<0.01);高能量水平组的LHR和FSHR mRNA表达量最高(P<0.05),能量来源对大卵泡卵丘-卵母细胞复合物中FSHR mRNA表达无显著影响,淀粉组上调LHR mRNA表达(P<0.05)。研究表明,提高日粮能量水平增加母猪排卵前大卵泡数量和卵母细胞成熟的比例,以淀粉为能量来源对卵泡数量没有显著影响,但提高了卵母细胞成熟的比例;能量影响卵泡数量和卵母细胞成熟可能与GDF-9、BMP-15和LHRF、SHR mRNA表达有关。Fifty-four Landrace× Yorkshire crossbred kg, were allotted to 3 × 2 factorial experiment to gilts, with initial body weight of （59±4.2） study the effects of dietary energy level and source on follicle development, oocyte maturation in vitro and growth differentiation factor 9 （GDF-9）, bone morphogenetic protein 15 （BMP-15）, LH receptor （LHR）, FSH receptor （FSHR） mRNA expression from cumulus-oocyte complexes （COCs） during the pre-ovulatory phase. Three energy levels were 87.5% （L）, 100% （M） and 112.5% （H） accounting for NRC （1998） recommended energy requirement, respectively ; two energy source were starch（S） and fat （F）. Gilts were slaughtered on day 19 after second estrus. The results showed that the number of large follicles were higher for gilts fed H （P〈0.05）, energy sources had no significant effect on number of follicles in gilts（P〉0.05） ; cumulus expansion and oocyte maturation were higher for gilts fed H （P〈 0.05）, energy sources had no significant effect on cumulus expansion（P〉 0.05） ; oocyte maturation were higher for gilts fed S than that fed F （P〈0.05） ; L energy level had higher GDF-9 and BMP-15 mRNA expression （P〈0.05）, energy sources had no effect on BMP-15mRNA expression, starch diets upregulated GDF-9 mRNA expression （P〈0.05）. GDF- 9 and BMP-15 mRNA expression were higher in COCs from small follicle than that from large follicle in all treatments （P〈0.01）. LHR and FSHR mRNA expression was higher in H （P〈 0.05）, energy sources had no significant effect on FSHR mRNA expression, however, starch diets increased LHR mRNA expression （P〈0.05）. The results of this study indicate that higher energy level could increase number of large follicle during the pre-ovulatory phase and oocyte maturation, although energy sources had no significant effect on number of follicles, starch diets increase oocytes maturation. The effects of energy level and source on number of follicle and oocyte maturation mayb

关 键 词：能量水平 能量来源 后备母猪 卵母细胞质量 基因表达 

分 类 号：S828.2[农业科学—畜牧学]