催乳素及其受体在青年公羊驼皮肤中分布的研究  

The Study on the Distribution of PRL and Its Receptor in Skin of Young Male Alpaca(Lama Pacos)

作　　者：赫晓燕[1] 朱芷葳[1] 游蓉丽[1] 郝欢庆[1] 范瑞文[1] 高莉[1] 曹靖[1] 董彦君[1] 董常生[1] 

机构地区：[1]山西农业大学动物科技学院,太谷030801

出　　处：《畜牧兽医学报》2008年第12期1765-1771,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：引进国际农业先进科学技术项目(948项目)(2003-Z86);山西省科技攻关项目(200603106020080311034)

摘　　要：羊驼是一种经济价值很高的毛用动物,研究包括激素在内的各种因素对羊驼毛生长的调控极为重要。作者采用基因芯片杂交技术发现青年公羊驼皮肤中不仅存在PRL及其受体基因,而且还存在PRL相关蛋白基因,提示羊驼皮肤是PRL的一个垂体外合成部位,同时羊驼皮肤还可以合成PRL相关蛋白及PRL受体;通过荧光定量分析比较,认为羊驼皮肤合成的PRL是极少量的,垂体是PRL合成的主要部位;通过免疫组织化学技术确定PRL及其受体蛋白主要分布在羊驼毛囊上皮根鞘、表皮、皮脂腺等,提示PRL对羊驼皮肤及其衍生物可能以内分泌、自分泌和旁分泌的方式发挥作用。Alpaca is one of the most important hair-producing animals. However, the genetic regulation of the growth of alpaca hair is largely unknown. Here, we report a surprising finding that prolactin （PRL）, PRL receptor （PRL-R） and other PRL related genes were expressed in the male alpaca skin. The expression levels of PRL in the skin were relatively lower than that in pituitary gland. The PRL and its receptor protein were detected in root sheath of hair follicles, epidermal and sebaceous gland of alpaca. The expression of PRL and PRL-R in the male alpaca suggested that PRL may regulate the growth of alpaca hair through an unknown mechanism in addition to its established role in milk production.

关 键 词：青年公羊驼 皮肤 PRL PRL相关蛋白 PRL受体 

分 类 号：S852.2[农业科学—基础兽医学]